Kacorzyk Radosław, Jakiela Bogdan, Maciejska Alicja, Węgrzyn Agnieszka S, Ćmiel Adam, Sanak Marek, Mastalerz Lucyna
2nd Department of Internal Medicine, Jagiellonian University Medical College, Kraków, Poland.
Doctoral School of Medical and Health Sciences, Jagiellonian University Medical College, Kraków, Poland.
Eur J Immunol. 2025 Aug;55(8):e70020. doi: 10.1002/eji.70020.
Previous studies confirmed increased group 2 innate lymphoid cell (ILC2s) count in nasal scrapings, alongside a reduced blood ILC2 count, in patients with nonsteroidal anti-inflammatory drug-exacerbated respiratory disease (N-ERD) after intranasal administration of cyclooxygenase-1 inhibitors. This study aimed to assess the role of blood and sputum ILCs in N-ERD patients during oral aspirin-induced bronchospasm and to compare patients with eosinophilic and noneosinophilic airway inflammatory phenotypes of asthma. Induced sputum, blood, and urine samples were collected in 24 patients with confirmed N-ERD at baseline and during aspirin-induced bronchospasm. Sputum and blood ILC counts were evaluated using flow cytometry. There was a significant increase in blood ILC1 count (p < 0.001) and percentage (p = 0.003) during aspirin-induced bronchospasm. No significant changes in sputum ILCs were observed, but the number of detected ILCs was very low. There was a significant reduction in induced sputum supernatant (ISS) levels of prostaglandins PGE (p = 0.004) and PGD (p = 0.045), leukotriene B (p = 0.045), and 15-oxo-eicosatetraenoic acid (p = 0.045) during aspirin-induced bronchospasm. Blood ILC1 count is increased during oral aspirin-induced bronchospasm in patients with N-ERD, but sputum ILC count at baseline is very low. Therefore, no reliable changes in sputum ILC counts can be detected during bronchospasm.
先前的研究证实，在鼻内给予环氧化酶-1抑制剂后，非甾体抗炎药加重的呼吸道疾病（N-ERD）患者的鼻拭子中第2组固有淋巴细胞（ILC2s）计数增加，同时血液中ILC2计数减少。本研究旨在评估血液和痰液中的ILC在N-ERD患者口服阿司匹林诱发支气管痉挛期间的作用，并比较嗜酸性粒细胞性和非嗜酸性粒细胞性气道炎症表型的哮喘患者。在24例确诊的N-ERD患者的基线期和阿司匹林诱发支气管痉挛期间收集诱导痰、血液和尿液样本。使用流式细胞术评估痰液和血液中的ILC计数。在阿司匹林诱发支气管痉挛期间，血液中ILC1计数（p < 0.001）和百分比（p = 0.003）显著增加。未观察到痰液中ILC有显著变化，但检测到的ILC数量非常少。在阿司匹林诱发支气管痉挛期间，诱导痰上清液（ISS）中前列腺素PGE（p = 0.004）、PGD（p = 0.045）、白三烯B（p = 0.045）和15-氧代-二十碳四烯酸（p = 0.045）的水平显著降低。N-ERD患者口服阿司匹林诱发支气管痉挛期间血液中ILC1计数增加，但基线时痰液中ILC计数非常低。因此，在支气管痉挛期间无法检测到痰液中ILC计数的可靠变化。
