Abuassaf Razan Ataallah, Al-Jamal Fatima F, Abusara Osama H, Zihlif Malek, Deeb Ahmad A, Al-Rshaidat Mamoon M D
Department of Biological Sciences, School of Sciences, The University of Jordan, Amman, Jordan.
Department of Chemistry, Faculty of Science, Isra University, Amman, Jordan.
PeerJ. 2025 Jul 31;13:e19735. doi: 10.7717/peerj.19735. eCollection 2025.
Marine sponges are known for their rich variety of secondary metabolites, many of which show potential for pharmaceutical applications. In this study, three deep-sea sponge species-, and -were identified using DNA barcoding, and their ethanolic extracts were tested for antibacterial activity. The extracts were evaluated against Gram-positive (, and methicillin-resistant , MRSA) and Gram-negative bacteria (, and using the agar well diffusion method. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were also determined. Among the extracts, exhibited the most potent antibacterial activity, with inhibition zones ranging from six to 21 mm against gram-positive bacteria and low MIC/MBC values from 0.25 to three mg/ml. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis of revealed the presence of bioactive compounds such as gallic acid, caffeic acid, bolinaquinone, dactyloquinone, and others, which are known for their antimicrobial properties. These findings suggest that has promising antibacterial properties that could be valuable in combating antimicrobial resistance.
海洋海绵以其丰富多样的次生代谢产物而闻名，其中许多具有药物应用潜力。在本研究中，利用DNA条形码鉴定了三种深海海绵物种，并测试了它们的乙醇提取物的抗菌活性。采用琼脂孔扩散法，针对革兰氏阳性菌（金黄色葡萄球菌、表皮葡萄球菌和耐甲氧西林金黄色葡萄球菌，MRSA）和革兰氏阴性菌（大肠杆菌、肺炎克雷伯菌和铜绿假单胞菌）对提取物进行了评估。还测定了最低抑菌浓度（MIC）和最低杀菌浓度（MBC）。在提取物中，[提取物名称]表现出最强的抗菌活性，对革兰氏阳性菌的抑菌圈范围为6至21毫米，MIC/MBC值低至0.25至3毫克/毫升。对[提取物名称]的液相色谱 - 质谱联用（LC-MS/MS）分析表明存在生物活性化合物，如没食子酸、咖啡酸、波林醌、指状醌等，它们以其抗菌特性而闻名。这些发现表明[提取物名称]具有有前景的抗菌特性，在对抗抗菌耐药性方面可能具有重要价值。
