Terunuma Atsushi, Chiu Chun Kai, Sekine Tatsuya, Takane Tsubasa, Sekine Yoshihisa, Ashiba Keisuke, Terunuma Hiroshi
N2 Clinic Yotsuya, Tokyo, Japan.
Biotherapy Institute of Japan, Tokyo, Japan.
J Stem Cells Regen Med. 2025 May 29;21(1):25-30. doi: 10.46582/jsrm.2101005. eCollection 2025.
Mesenchymal stem cells (MSCs) suppress inflammation and promote tissue repair via paracrine factors. MSC-conditioned medium (MSC-CM), rich in these factors, shows promise as a cell-free therapy. This study explored the protein profiles of MSC-CMs from different human tissues (dental pulp, adipose tissue, umbilical cord, and placenta) to assess variations and therapeutic potential.
MSCs were cultured from dental pulp, adipose tissue, umbilical cord, and placenta, and MSC-CMs were collected. Proteomic analysis using LC-MS/MS identified and quantified proteins, followed by bioinformatic analysis.
A total of 924 secreted proteins were identified in MSC-CMs from the four tissue sources. Extracellular matrix (ECM) signatures were prominent across all MSC-CMs. MSC-CM from adipose tissue had the highest levels of skin care-related proteins. Neuronal growth-related proteins were most abundant in umbilical cord and placental MSC-CMs, while wound healing proteins were prominent in dental pulp MSC-CM.
MSC-CMs from different tissues exhibit distinct protein profiles, while sharing common ECM signatures. These findings suggest that MSC-CM could be used for specific applications such as neurodegenerative diseases and wound healing, depending on the tissue source. Further in vivo research is needed to explore their clinical relevance.
间充质干细胞（MSCs）通过旁分泌因子抑制炎症并促进组织修复。富含这些因子的MSC条件培养基（MSC-CM）显示出作为无细胞疗法的潜力。本研究探索了来自不同人体组织（牙髓、脂肪组织、脐带和胎盘）的MSC-CM的蛋白质谱，以评估其差异和治疗潜力。
从牙髓、脂肪组织、脐带和胎盘中培养MSCs，并收集MSC-CM。使用液相色谱-串联质谱（LC-MS/MS）进行蛋白质组学分析以鉴定和定量蛋白质，随后进行生物信息学分析。
在来自四种组织来源的MSC-CM中总共鉴定出924种分泌蛋白。细胞外基质（ECM）特征在所有MSC-CM中都很突出。来自脂肪组织的MSC-CM中与皮肤护理相关的蛋白质水平最高。与神经元生长相关的蛋白质在脐带和胎盘MSC-CM中最为丰富，而伤口愈合相关蛋白质在牙髓MSC-CM中很突出。
来自不同组织的MSC-CM表现出不同的蛋白质谱，同时共享共同的ECM特征。这些发现表明，根据组织来源，MSC-CM可用于特定应用，如神经退行性疾病和伤口愈合。需要进一步的体内研究来探索它们的临床相关性。
