南瓜（杜申南瓜）中对南瓜曲叶中国病毒（SLCCNV）和葫芦褪绿黄化病毒（CCYV）的新型抗性来源鉴定及SLCCNV抗性的遗传分析。

Identification of novel sources of resistance to squash leaf curl China virus (SLCCNV) and cucurbit chlorotic yellows virus (CCYV) and inheritance analysis of SLCCNV resistance in pumpkin ( Duchesne ex Poir.).

作者信息

Rout Bichhinna Maitri, Sureja Amish Kumar, Subramanian S, Krishnan S Gopala, Roy Anirban, Dash Prasanta Kumar, Das Munshi Anilabha

机构信息

Division of Vegetable Science, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.

The Graduate School, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.

出版信息

Physiol Mol Biol Plants. 2025 Jun;31(6):913-929. doi: 10.1007/s12298-025-01613-2. Epub 2025 Jul 9.

DOI:10.1007/s12298-025-01613-2

PMID:40756441

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12314156/

Abstract

UNLABELLED

Pumpkin ( Duchesne ex. Poir) is susceptible to various viral infections which significantly hinder its production, impacting agricultural sustainability and food security. The susceptibility of fifty four pumpkin genotypes to squash leaf curl China virus (SLCCNV) and cucurbit chlorotic yellows virus (CCYV) was assessed over two consecutive seasons (2021 rainy and 2022 spring-summer) and in 2023 (spring-summer), specifically for only SLCCNV, through screening in an insect-proof net house under whitefly inoculation conditions. Disease progression was evaluated through the vulnerability index (VI) and the area under the disease progress curve (AUDPC). During 2021 and 2022, on the basis of challenge inoculation studies, highly resistant genotypes (DPU-41, DPU-43, DPU-133, and DPU-105) consistently presented no symptoms and no PCR amplification of SLCCNV or CCYV. Furthermore, during 2023, four genotypes (DPU-41, DPU-43, DPU-133 and DPU-105) presented high resistance levels (VI = 0) to SLCCNV. VI and AUDPC exhibited a significant positive correlation (> 0.96) for whitefly-mediated inoculation screening during 2021 and 2022. Quantitative polymerase chain reaction (qPCR) analysis revealed no detectable SLCCNV load in the highly resistant (DPU-41, DPU-43, DPU-133, and DPU-105) and resistant (DPU-101, and DPU-129) genotypes, with cycle threshold (Ct) values indicating the absence of the virus. Furthermore, inheritance studies involving susceptible and resistant genotypes across six generations (P, P, F, F, B, B) revealed a single dominant gene governing resistance to SLCCNV in the DPU-41 and DPU-43 genotypes. The present findings are the first to reveal the single dominant gene inheritance of resistance to SLCCNV in pumpkin. This study contributes to understanding resistance in pumpkin genotypes against SLCCNV and CCYV, offering a foundation for breeding programs focused on producing resistant varieties.

SUPPLEMENTARY INFORMATION

The online version contains supplementary material available at 10.1007/s12298-025-01613-2.

摘要

未标注

南瓜（杜谢讷氏种，波伊氏命名）易受多种病毒感染，这严重阻碍了其产量，影响农业可持续性和粮食安全。在连续两个季节（2021年雨季和2022年春夏季）以及2023年（春夏季），通过在防虫网室内粉虱接种条件下进行筛选，评估了54种南瓜基因型对中国南瓜曲叶病毒（SLCCNV）和葫芦科褪绿黄化病毒（CCYV）的易感性，2023年只针对SLCCNV进行评估。通过易感性指数（VI）和病情进展曲线下面积（AUDPC）评估疾病进展情况。在2021年和2022年，基于挑战接种研究，高抗基因型（DPU - 41、DPU - 43、DPU - 133和DPU - 105）始终未出现症状，且未检测到SLCCNV或CCYV的PCR扩增产物。此外，在2023年，四种基因型（DPU - 41、DPU - 43、DPU - 133和DPU - 105）对SLCCNV表现出高抗性水平（VI = 0）。在2021年和2022年粉虱介导接种筛选中，VI和AUDPC呈现显著正相关（> 0.96）。定量聚合酶链反应（qPCR）分析显示，在高抗（DPU - 41、DPU - 43、DPU - 133和DPU - 105）和抗性（DPU - 101和DPU - 1）基因型中未检测到SLCCNV载量，循环阈值（Ct）值表明不存在该病毒。此外，涉及六个世代（P、P、F、F、B）的感病和抗病基因型的遗传研究表明，DPU - 41和DPU - 43基因型中存在一个控制对SLCCNV抗性的显性基因。本研究结果首次揭示了南瓜中对SLCCNV抗性的单显性基因遗传。该研究有助于了解南瓜基因型对SLCCNV和CCYV的抗性，为专注于培育抗性品种的育种计划提供了基础。