Qin Hongtao, Abhinandan Kumar, Wang Min, Chen Huan, Zhang Xue, Li Lanlan, Zhou Zhengwenyang, Wang Suhui, Zhao Chaoning, Mu Wendi, Yuan Yongxue, Wu Ying, Li Yuhua, Samuel Marcus A, Lan Xingguo
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, No. 26 Hexing Road, Xiangfang District, Harbin, Heilongjiang 150040, China.
Department of Biological Sciences, University of Calgary, 2500 University Drive N.W., Calgary, Alberta T2N 1N4, Canada.
Hortic Res. 2025 Jun 11;12(9):uhaf147. doi: 10.1093/hr/uhaf147. eCollection 2025 Sep.
Self-incompatibility (SI) is a complex molecular mechanism in flowering plants that prevents self-fertilization and promotes outcrossing. We conducted metabolome analysis of ornamental kale ( var. ) pistils following SI and compatible pollination (CP), revealing significant alterations in lipid metabolism, particularly the accumulation of free fatty acid (FFA) metabolites during CP. Treatment of stigmas with acetyl-CoA and malonyl-CoA, key precursors in fatty acid (FA) synthesis, broke down SI and enhanced CP. Conversely, inhibiting acetyl-CoA carboxylase (ACCase), the rate-limiting enzyme in FA synthesis, significantly reduced compatible pollen attachment and tube growth, highlighting the critical role of FA metabolism in mediating pollination success. We identified a novel interaction between the FERONIA (BoFER) receptor kinase and the biotin carboxyl carrier protein 1 (BoBCCP1), a subunit of the ACCase complex. Suppressing the expression of in the stigma reduced CP response, suggesting that the FER-BCCP1 module may play a crucial role in regulating FA biosynthesis and determining the outcome of pollen-stigma interactions. Our findings provide new insights into the identification of key metabolic pathways and signaling modules controlling pollen-stigma interactions, and offer a valuable resource for the targeted improvement of crop breeding.
自交不亲和性（SI）是开花植物中一种复杂的分子机制，可防止自花受精并促进异花授粉。我们对观赏羽衣甘蓝（变种）雌蕊在自交不亲和授粉（SI）和亲和授粉（CP）后的代谢组进行了分析，发现脂质代谢发生了显著变化，尤其是在亲和授粉期间游离脂肪酸（FFA）代谢物的积累。用脂肪酸（FA）合成的关键前体乙酰辅酶A和丙二酰辅酶A处理柱头，打破了自交不亲和性并增强了亲和授粉。相反，抑制FA合成中的限速酶乙酰辅酶A羧化酶（ACCase），显著降低了亲和花粉的附着和花粉管生长，突出了FA代谢在介导授粉成功中的关键作用。我们鉴定了FERONIA（BoFER）受体激酶与生物素羧基载体蛋白1（BoBCCP1）之间的一种新相互作用，BoBCCP1是ACCase复合体的一个亚基。抑制柱头中 的表达降低了亲和授粉反应，表明FER-BCCP1模块可能在调节FA生物合成和决定花粉-柱头相互作用结果中起关键作用。我们的研究结果为识别控制花粉-柱头相互作用的关键代谢途径和信号模块提供了新见解，并为有针对性地改良作物育种提供了宝贵资源。
