Mersakova Sandra, Marcinek Juraj, Brany Dusan, Chodelkova Olga, Vorcak Martin, Cermak Martin, Poturnajova Martina, Kozovska Zuzana, Skovierova Henrieta, Novakova Slavomira, Mitruskova Barbora, Loderer Dusan, Grendar Marian, Holubekova Veronika, Hornakova Andrea, Melegova Jana, Brozmanova Mariana, Palkoci Blazej, Vojtko Martin, Kycina Roman, Pindura Miroslav, Janik Jan, Mikolajcik Peter, Gabonova Eva, Laca Ludovit, Nicodemou Andreas, Danisovic Lubos, Plank Lukas, Halasova Erika, Mraz Marek, Matuskova Miroslava, Lasabova Zora, Kalman Michal, Strnadel Jan
Biomedical Centre Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Mala Hora 4C, 036 01, Martin, Slovakia.
Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, and University Hospital Martin, Comenius University in Bratislava, 036 01, Martin, Slovakia.
Hum Cell. 2025 Aug 4;38(5):137. doi: 10.1007/s13577-025-01256-1.
Metastatic colorectal cancer is one of the most critical causes of cancer-related dead in patients suffering this type of malignancy. As the liver metastases are found in almost 25-50% of all colorectal cancer patients, there is an urgent need for suitable in vitro and in vivo models. To the best of our knowledge, only a limited number of well-described 2D or 3D organoid/spheroid cell lines originated from human liver metastases was reported for this type of malignancy and submitted to ATCC and other repository centers for general use. Here, we present a novel, well-characterized cell line Coala that was derived directly from liver metastasis of patient with colorectal cancer. Coala cancer cell line was derived by 2D and 3D culture technology and can be, therefore, cultured as standard 2D cell line or as 3D spheroid culture. When transplanted into athymic mice, Coala cell line forms fast growing tumors with histological features similar to original tumor and expressed CDX2, SATB2, and CK20. Whole exome sequencing analysis of Coala cell line showed the presence of pathogenic mutations in C8B, PRKCQ, IRGM, FGFR4, POLR1C, APC and TP53 genes. As verified by antibody array chip analysis, Coala cells express Snail, Pdx-1, FoxA2, and E-cadherin. Our cell line represents a new, well characterized in vitro research tool for cancer research and will be available to researchers in the field via biorepository centers.
转移性结直肠癌是这类恶性肿瘤患者癌症相关死亡的最关键原因之一。由于在几乎25%-50%的结直肠癌患者中都发现有肝转移，因此迫切需要合适的体外和体内模型。据我们所知，对于这类恶性肿瘤，仅报道了有限数量的源自人肝转移灶的、描述详尽的二维或三维类器官/球体细胞系，并提交给了美国典型培养物保藏中心及其他保藏中心以供广泛使用。在此，我们展示了一种新的、特征明确的细胞系Coala，它直接源自一名结直肠癌患者的肝转移灶。Coala癌细胞系通过二维和三维培养技术获得，因此既可以作为标准的二维细胞系培养，也可以作为三维球体培养。将Coala细胞系移植到无胸腺小鼠体内时，会形成生长迅速的肿瘤，其组织学特征与原发肿瘤相似，并表达CDX2、SATB2和CK20。对Coala细胞系进行的全外显子组测序分析显示，C8B、PRKCQ、IRGM、FGFR4、POLR1C、APC和TP53基因存在致病性突变。经抗体阵列芯片分析验证，Coala细胞表达Snail、Pdx-1、FoxA2和E-钙黏蛋白。我们的细胞系代表了一种新的、特征明确的用于癌症研究的体外研究工具，将通过生物样本库中心提供给该领域的研究人员。
