Ajayi S T, Smith B F, LeFlore W B
Cytobios. 1985;44(175):19-24.
Freshly shed gravid proglottids from a three-month-old infection of Hydatigera taeniaeformis collected from the faecal droppings of infected cats were used for this study. They were treated for transmission electron microscopy (TEM) followed by incubation using the lead precipitate method. Control sections were incubated in a substrate-free medium, a substrate medium containing 1.0 mM sodium fluoride (NaF) (an inhibitor), and the last sections were denatured at 90 degrees C for 1 min prior to incubation. Intensive alkaline phosphatase activity in the embryophoric blocks and the outer embryophoric membrane was revealed. The reaction products were also indicated in the oncospheral membrane. However, no enzyme activity was seen in any other part of the egg. The enzyme was also absent in the control sections. The presence of alkaline phosphatase activity in the outer embryophoric and oncospheral membranes suggested that this enzyme may be involved in carbohydrate metabolism and nutritional absorption, and also may play a role in the transport of nutrients and other substances from the adult to the developing embryo, respectively.
本研究使用从感染猫的粪便中收集的、来自感染绦虫三个月的泡状带绦虫新鲜脱落的妊娠节片。将其进行透射电子显微镜（TEM）处理，然后采用铅沉淀法进行孵育。对照切片在无底物培养基、含有1.0 mM氟化钠（NaF）（一种抑制剂）的底物培养基中孵育，最后一部分切片在孵育前于90℃变性1分钟。在胚膜块和胚膜外层发现了强烈的碱性磷酸酶活性。反应产物也出现在六钩蚴膜中。然而，在虫卵的任何其他部位均未观察到酶活性。对照切片中也未发现该酶。胚膜外层和六钩蚴膜中碱性磷酸酶活性的存在表明，这种酶可能分别参与碳水化合物代谢和营养吸收，也可能在营养物质和其他物质从成虫向发育中的胚胎运输中发挥作用。
