Zhu Xiaoxue, Zou Anlai, Zhao Mengliu, Hou Jinjie, Xianyu Yunlei
Department of Clinical Laboratory of Sir Run Run Shaw Hospital, College of Biosystems Engineering and Food Science, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Key Laboratory of Precision Medicine in Diagnosis and Monitoring Research of Zhejiang Province, Sir Run Run Shaw Hospital, Hangzhou, 310016, China.
Adv Healthc Mater. 2025 Nov;14(30):e02050. doi: 10.1002/adhm.202502050. Epub 2025 Aug 5.
Oral ulcer, characterized by mucosal damage, is susceptible to bacterial infection, tissue inflammation, and slow healing. Traditional treatments for oral ulcers only target on single function, such as antimicrobial, anti-inflammatory, or wound-healing, limiting the therapeutic efficacy. In this work, a multifunctional nanotherapeutic approach is developed, integrating antibacterial, anti-inflammatory, and tissue-healing properties for the treatment of oral ulcers with bacterial infection. Outer membrane vesicles (OMVs) derived from probiotic Escherichia coli Nissle 1917 serve as biocompatible carriers to load platinum nanoparticles (Pt NPs) and curcumin (Cur), acting as multifunctional Cur@OMV-Pt (COP) nanocomposite for the treatment of oral ulcer. The probiotic OMVs enhance the delivery and promote tissue regeneration owing to their rich components and functional biomolecules. Pt NPs with peroxidase-like and catalase-like activities catalyze HO to generate reactive oxygen species and oxygen, exerting chemodynamic antibacterial therapy while enhancing photodynamic therapy of curcumin with anti-inflammatory effects. The COP nanocomposite effectively addresses the challenges of methicillin-resistant Staphylococcus aureus-infected oral ulcer by synergistically enhancing antibacterial, anti-inflammatory, and tissue-healing responses. This work presents a promising strategy for clinical applications in the treatment of oral ulcers and other bacterial infections, particularly in complex microenvironments.
口腔溃疡以黏膜损伤为特征，易受细菌感染、组织炎症影响，且愈合缓慢。口腔溃疡的传统治疗仅针对单一功能，如抗菌、抗炎或伤口愈合，限制了治疗效果。在这项工作中，开发了一种多功能纳米治疗方法，整合抗菌、抗炎和组织愈合特性来治疗伴有细菌感染的口腔溃疡。源自益生菌大肠杆菌Nissle 1917的外膜囊泡（OMV）作为生物相容性载体负载铂纳米颗粒（Pt NPs）和姜黄素（Cur），形成用于治疗口腔溃疡的多功能Cur@OMV-Pt（COP）纳米复合材料。益生菌OMV因其丰富的成分和功能性生物分子而增强递送并促进组织再生。具有类过氧化物酶和类过氧化氢酶活性的Pt NPs催化H₂O₂生成活性氧和氧气，发挥化学动力抗菌疗法，同时增强具有抗炎作用的姜黄素的光动力疗法。COP纳米复合材料通过协同增强抗菌、抗炎和组织愈合反应，有效应对耐甲氧西林金黄色葡萄球菌感染的口腔溃疡带来的挑战。这项工作为口腔溃疡及其他细菌感染的临床应用，特别是在复杂微环境中的应用，提供了一种有前景的策略。
