Li Lei, Li Haoze, Wang Junrong, Xie Yulin, Gao Minghong, Yang Zhongjun, Li Chunxia
Institute of Frontier Chemistry, School of Chemistry and Chemical Engineering, Shandong University, Qingdao, Shandong 266237, PR China.
Qilu Hospital (Qingdao), Cheeloo College of Medicine, Shandong University, 758 Hefei Road, Qingdao, Shandong 266035, PR China.
ACS Appl Mater Interfaces. 2024 Dec 11;16(49):67166-67177. doi: 10.1021/acsami.4c10720. Epub 2024 Nov 25.
Damaged skin compromises its ability to effectively prevent the invasion of harmful bacteria into the tissue, leading to bacterial infection of the wound and hindering the healing process. To address this challenge, we have developed a multifunctional asymmetric wound dressing (CuPt-Cur-ABC) that effectively addresses the lack of bactericidal activity and the release of active ingredients in conventional bacterial cellulose (BC), which can be employed to create a barrier of defense between the wound and its surrounding environment. Compared with BC, asymmetric bacterial cellulose (ABC) used starch as a pore-causing agent, forming holes of different sizes at the top and bottom, which enhanced the ability of ABC to load and moderate-release drugs. First, as-synthesized CuPt nanozymes with an octopod nanoframe structure had multiple enzymatic activities including peroxidase-like, catalase-like, and glutathione peroxidase-like activities. Then, CuPt and curcumin (Cur) were loaded into ABC under ultrasound. Under 808 nm laser irradiation, the nanocomposites possessed good photothermal properties. So the photothermal therapy combined with chemodynamic therapy and inherent antibacterial performance of Cur achieved 99.3% and 99.6% bactericidal efficacy against and , respectively. Moderate release of Cur can clear the excess reactive oxygen species and promote the polarization of macrophages toward the M2 type. experiments additionally confirmed that the constructed wound dressing achieved multiple functions, including effective antibacterial activity, reversing the inflammatory microenvironment, and promoting wound healing.
受损皮肤会损害其有效防止有害细菌侵入组织的能力，导致伤口细菌感染并阻碍愈合过程。为应对这一挑战，我们开发了一种多功能不对称伤口敷料（CuPt-Cur-ABC），它有效解决了传统细菌纤维素（BC）杀菌活性不足和活性成分释放问题，可用于在伤口及其周围环境之间建立防御屏障。与BC相比，不对称细菌纤维素（ABC）使用淀粉作为造孔剂，在顶部和底部形成不同大小的孔，增强了ABC负载和缓释药物的能力。首先，合成的具有八足纳米框架结构的CuPt纳米酶具有多种酶活性，包括过氧化物酶样、过氧化氢酶样和谷胱甘肽过氧化物酶样活性。然后，在超声作用下将CuPt和姜黄素（Cur）负载到ABC中。在808nm激光照射下，纳米复合材料具有良好的光热性能。因此，光热疗法联合化学动力疗法以及Cur的固有抗菌性能分别对金黄色葡萄球菌和大肠杆菌实现了99.3%和99.6%的杀菌效果。Cur的适度释放可以清除过量的活性氧，并促进巨噬细胞向M2型极化。实验进一步证实，构建的伤口敷料实现了多种功能，包括有效的抗菌活性、逆转炎症微环境和促进伤口愈合。
