An Yanlin, Zhang Lihong, Li Xueqi, Mi Xiaozeng, Qiao Dahe, Jing Tingting
School of Food Engineering, Moutai Institute, Luban Street, Renhuai 564502, Guizhou, China.
Guizhou Tea Research Institute, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou 550006, China.
Food Chem X. 2025 Jul 26;29:102830. doi: 10.1016/j.fochx.2025.102830. eCollection 2025 Jul.
Non-volatile metabolites and aroma substances are important quality components of tea plants, yet the quality characteristics of tea plants with special leaf colors still await further revelation. In this study, by integrating five analytical methods including widely targeted metabolomics, HS-SPME-GC-MS, and targeted metabolomics, a total of 1770 non-volatile metabolites were detected in ZK, NB, and ZH3, among which flavonoids, amino acids, and polyphenols were the main differential metabolites. The contents of total catechins, anthocyanins and amino acids in ZK, NB and ZH3 are 159.96, 122.03, 121.86 mg/g, 4.22, 4.77, 4.70 mg/g and 22.27, 60.01 and 61.83 mg/g, respectively. A total of 122 volatile compounds were identified, mainly including 36 alcohols, 20 aldehydes, 16 esters and 16 ketones. And 41, 36 and 37 key aroma compounds were identified in NB, ZH3 and ZK, respectively. These results provide valuable insights into understanding the quality basis of tea plants with different leaf colors.
非挥发性代谢产物和香气物质是茶树重要的品质成分，然而具有特殊叶色的茶树的品质特征仍有待进一步揭示。在本研究中，通过整合广泛靶向代谢组学、顶空固相微萃取-气相色谱-质谱联用（HS-SPME-GC-MS）和靶向代谢组学等五种分析方法，在ZK、NB和ZH3中总共检测到1770种非挥发性代谢产物，其中黄酮类、氨基酸和多酚是主要的差异代谢产物。ZK、NB和ZH3中总儿茶素、花青素和氨基酸的含量分别为159.96、122.03、121.86毫克/克，4.22、4.77、4.70毫克/克和22.27、60.01、61.83毫克/克。总共鉴定出122种挥发性化合物，主要包括36种醇类、20种醛类、16种酯类和16种酮类。并且在NB、ZH3和ZK中分别鉴定出41种、36种和37种关键香气化合物。这些结果为理解不同叶色茶树的品质基础提供了有价值的见解。
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