Godoy Fernanda M R, Paggi Gecele M, Lorenz Aline P, Ramos Jeferson V, Franco Daniel G, Calarge Fernando M L, Garcia Nayara F L, Urquiza Marcus V S, Schiavo Jolimar A, Almeida Nalvo F, Brasil Marivaine S
Microbiology and Genetics Laboratory, Pantanal Campus, Federal University of Mato Grosso do Sul, Corumbá, Mato Grosso do Sul, Brazil.
Ecology and Evolutionary Biology Laboratory, Institute of Biosciences, Federal University of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.
Int J Microbiol. 2025 Jul 28;2025:6374781. doi: 10.1155/ijm/6374781. eCollection 2025.
Knowledge about the diversity and distribution of microorganisms in natural environments is essential for understanding the dominant microbial groups and predicting their ecological functions. This study is aimed at describing the bacteriome diversity in soils associated with bromeliads in the Brazilian Pantanal region, utilizing genomic approaches. We analyzed the 16S rRNA gene from soil environmental DNA (eDNA) samples linked to and (Bromeliaceae), which inhabit ironstone outcrops in the Pantanal. The analysis revealed Ktedonobacteraceae as the most abundant bacterial group, showing a mean relative abundance of 22.8% ± 15.5% in and 33.5% ± 18.4% in soils. Other highly abundant families were Chthoniobacteraceae and Pyrinomonadaceae, each exceeding 14.5% mean abundance. Despite the similarities in bacteriome composition between the bromeliads, beta-diversity analysis revealed phylogenetic distinctions across localities. The São João and Vale do Paraíso Farms, which experience the highest human impact from livestock farming, showed considerable differences, with 25 and 13 exclusive taxa, respectively. The environmental stresses of ironstone outcrops, such as high insolation and thermal variation, likely favor specific taxa adapted to these conditions. Understanding the bacteriome diversity in these unique habitats is crucial for promoting sustainable use and conserving the Pantanal's biodiversity.
了解自然环境中微生物的多样性和分布对于认识优势微生物群体并预测其生态功能至关重要。本研究旨在利用基因组学方法描述巴西潘塔纳尔地区与凤梨科植物相关的土壤中的细菌群落多样性。我们分析了与生长在潘塔纳尔地区铁矿石露头的红凤梨和小红凤梨相关的土壤环境DNA（eDNA）样本中的16S rRNA基因。分析结果显示，鞘脂杆菌科是最丰富的细菌类群，在红凤梨土壤中的平均相对丰度为22.8%±15.5%，在小红凤梨土壤中的平均相对丰度为33.5%±18.4%。其他高度丰富的科是嗜铁杆菌科和梨单胞菌科，它们的平均丰度均超过14.5%。尽管凤梨科植物之间的细菌群落组成存在相似性，但β多样性分析揭示了不同地点之间的系统发育差异。受畜牧业人类影响最大的圣若昂农场和帕拉伊索谷农场表现出相当大的差异，分别有25个和13个独特的分类单元。铁矿石露头的环境压力，如高日照和热变化，可能有利于适应这些条件的特定分类单元。了解这些独特栖息地中的细菌群落多样性对于促进可持续利用和保护潘塔纳尔的生物多样性至关重要。
