Jin Huadan, Fu Yihan, Zhao Dan, Wang Liye, Wang Yaoyan, Hu Rongqing, Fu Renjie
Oral Health Prev Dent. 2025 Aug 5;23:427-433. doi: 10.3290/j.ohpd.c_2153.
Peri-implantitis (PI) is the primary cause of implant failure, and genetic susceptibility significantly influences its development. This study investigated the association between the MMP-8 rs11225395 polymorphism and PI in the Chinese Han population.
In this study, 140 Chinese Han patients diagnosed with PI and 156 healthy implant controls were included. Polymerase chain reaction (PCR) was employed to detect the MMP-8 rs11225395 polymorphism, and the Hardy-Weinberg equilibrium test was conducted to assess the representativeness of the samples. Additionally, enzyme-linked immunosorbent assay (ELISA) was utilised to quantify the expression levels of MMP-8, and logistic regression analysis was performed to identify independent risk factors for PI disease.
There were statistically significant differences in the genotype and allele distributions of the MMP-8 rs11225395 locus between PI patients and the control group. Rs11225395 T allele was significantly associated with an increased risk of PI, particularly for individuals with the TC/TT genotype who exhibited higher susceptibility to the disease. Periodontal status indicators differed markedly among PI patients with different genotypes. Factors such as plaque index, brushing daily, probing pocket depth (PPD), clinical attachment loss (CAL), and MMP-8 rs11225395 polymorphism played crucial roles in PI risk. Additionally, MMP-8 expressions were upregulated in PI patients. Specifically, at the rs11225395 locus, individuals with the TC/TT genotype showed a significantly higher relative expression level of MMP-8.
The MMP-8 rs11225395 polymorphism was significantly associated with genetic susceptibility to PI.
种植体周炎（PI）是种植体失败的主要原因，遗传易感性对其发展有显著影响。本研究调查了中国汉族人群中基质金属蛋白酶8（MMP - 8）基因rs11225395多态性与种植体周炎之间的关联。
本研究纳入了140例诊断为种植体周炎的中国汉族患者和156例健康种植体对照者。采用聚合酶链反应（PCR）检测MMP - 8 rs11225395多态性，并进行哈迪 - 温伯格平衡检验以评估样本的代表性。此外，利用酶联免疫吸附测定（ELISA）定量MMP - 8的表达水平，并进行逻辑回归分析以确定种植体周炎疾病的独立危险因素。
种植体周炎患者与对照组之间MMP - 8 rs11225395位点的基因型和等位基因分布存在统计学显著差异。rs11225395 T等位基因与种植体周炎风险增加显著相关，特别是对于TC/TT基因型个体，其对该疾病表现出更高的易感性。不同基因型的种植体周炎患者牙周状况指标存在明显差异。菌斑指数、每日刷牙情况、探诊深度（PPD）、临床附着丧失（CAL）和MMP - 8 rs11225395多态性等因素在种植体周炎风险中起关键作用。此外，种植体周炎患者中MMP - 8表达上调。具体而言，在rs11225395位点，TC/TT基因型个体的MMP - 8相对表达水平显著更高。
MMP - 8 rs11225395多态性与种植体周炎的遗传易感性显著相关。
