Cintora-Mairal Carmen, de la Rosa José Manuel Ortiz, Pina-Vaz Cidália, Martín-Gutiérrez Guillermo, Lepe José Antonio
Unidad de Enfermedades Infecciosas, Microbiología y Parasitología Clínica, Hospital Universitario Virgen del Rocío, Seville, Spain.
Instituto de Biomedicina de Sevilla (IBiS), Hospital Universitario Virgen del Rocío, CSIC/Universidad de Sevilla, Seville, Spain.
Sci Rep. 2025 Aug 5;15(1):28649. doi: 10.1038/s41598-025-12137-w.
Pseudomonas aeruginosa is a critical priority pathogen due to its high resistance rates and limited treatment options. Rapid antimicrobial susceptibility testing (rAST) is essential to optimize therapy, particularly because resistance mechanisms in P. aeruginosa often arise from adaptive genetic expression rather than specific resistance genes. FASTinov, a flow cytometry-based rAST system, was developed to address this challenge by providing phenotypic susceptibility results within hours. In this study, we assessed FASTinov's performance for rapid antimicrobial susceptibility testing (rAST) in 100 P. aeruginosa strains, using broth microdilution as the reference method. For clinical relevance, we also included the MicroScan WalkAway system as a comparator. FASTinov demonstrated a categorical agreement of 99% across the tested antibiotics, with particularly strong agreement for cephalosporins. Notably, FASTinov significantly reduced the time to results, delivering susceptibility data in an average of 3.15 h (CI 95% 3.13-3.17), compared to 28.88 h (CI 95% 20.71-37.04) for standard methods. Additionally, the system exhibited lower rates of major and very major errors compared to MicroScan, further reinforcing its reliability. These findings highlight FASTinov as a promising rAST system capable of providing rapid and accurate susceptibility profiles directly from bacterial colonies. Further research is warranted to evaluate its clinical impact on patient outcomes and cost-effectiveness.
铜绿假单胞菌因其高耐药率和有限的治疗选择而成为关键优先病原体。快速抗菌药物敏感性测试（rAST）对于优化治疗至关重要，特别是因为铜绿假单胞菌的耐药机制通常源于适应性基因表达而非特定的耐药基因。FASTinov是一种基于流式细胞术的rAST系统，旨在通过在数小时内提供表型药敏结果来应对这一挑战。在本研究中，我们以肉汤微量稀释法作为参考方法，评估了FASTinov在100株铜绿假单胞菌中进行快速抗菌药物敏感性测试（rAST）的性能。为了具有临床相关性，我们还纳入了MicroScan WalkAway系统作为比较对象。FASTinov在所有测试抗生素中的分类一致性为99%，对头孢菌素的一致性尤其高。值得注意的是，FASTinov显著缩短了获得结果的时间，平均3.15小时（95%置信区间3.13 - 3.17）即可提供药敏数据，而标准方法则需要28.88小时（95%置信区间20.71 - 37.04）。此外，与MicroScan相比，该系统的主要和非常主要错误发生率更低，进一步增强了其可靠性。这些发现凸显了FASTinov作为一种有前景的rAST系统，能够直接从细菌菌落提供快速准确的药敏谱。有必要进一步研究以评估其对患者预后的临床影响和成本效益。
