Centre for Biotechnology, Anna University, Chennai, 600 025, India.
Department of Electronic Systems Engineering, Indian Institute of Science, Bangalore, 560 012, India.
Sci Rep. 2020 Mar 4;10(1):4058. doi: 10.1038/s41598-020-60717-9.
We recently reported a sugar-induced bacterial release of 13-Docosenamide and its ability to quench fluorescein. This simple handle to monitor bacterial growth is readily applicable to develop a quicker antibiotic sensitivity testing method along with a low-cost field-use optical instrumentation. Conditions were standardized to perform this new procedure in the most preferred and CLSI-recommended microdilution format in 12-well strips. A simple and portable optoelectronic prototype was used to capture the image and read the fluorescence signal of the culture medium of the 12-well strips. This new Fluorescence Quenching Method along with the device enabled the choice of the right antibiotic within 8 h of sample collection from the patient. It was compliant to the Clinical Laboratory Standard Institute's quality control guidelines. Clinical assessment of the method using 440 urine samples from Urinary Tract Infection patients against 21 routinely used antibiotics showed a 94.3% match with the results of the Standard Disk Diffusion method. This new method saves the precious time taken for and the cost of antibiotic susceptibility testing for quicker and effective treatment with better compliance.
我们最近报道了一种糖诱导的细菌释放 13-二十二碳烯酰胺及其淬灭荧光素的能力。这种简单的方法可以用来监测细菌的生长，很容易开发出一种更快的抗生素敏感性测试方法，并结合低成本的现场使用光学仪器。条件已经标准化，以便在最受欢迎和 CLSI 推荐的 12 孔条微量稀释格式中执行此新程序。使用简单便携的光电原型来捕获图像并读取 12 孔条培养物的荧光信号。这种新的荧光猝灭方法以及该设备能够在从患者采集样本后的 8 小时内选择合适的抗生素。它符合临床实验室标准协会的质量控制指南。该方法使用 440 份尿路感染患者的尿液样本对 21 种常用抗生素进行临床评估，与标准圆盘扩散方法的结果吻合度达到 94.3%。这种新方法节省了抗生素敏感性测试所需的宝贵时间和成本，可实现更快、更有效的治疗，提高了患者的依从性。
