Wang Jun, Wang Bo, Zhang Yun, Wu Li-Qiang
Department of Hematology, The First Affiliated Hospital of Zhejiang Chinese Medical University, No.54, Youdian Road, Shangcheng District, Hangzhou, 310006, Zhejiang, China.
Sci Rep. 2025 Aug 5;15(1):28640. doi: 10.1038/s41598-025-14105-w.
To investigate the mechanism and potential targets of Yiqi Ziyin (YQZY) for treating immune thrombocytopenia (ITP). Prednisone and YQZY were orally administered to ITP mice. Post-treatment, blood samples were taken to evaluate the blood picture. The spleen was obtained to observe histomorphological changes and calculate the spleen index. The active components and predicted related targets of YQZY were obtained based on the TCMSP database. The ITP-related targets were screened by GeneCards and DisGeNET databases. Then, functional enrichment analyses were conducted to examine the potential regulatory pathways of YQZY in ITP, which was validated by western blot assay. Additionally, three different networks were constructed to identify the predominant targets and ingredients of YQZY in the ITP treatment, followed by molecular docking analysis. YQZY significantly upregulated platelet counts and improved the other blood index abnormalities caused by anti-platelet serum injection. Totally 60 active ingredients were acquired, and 85 common targets of active ingredients and ITP. The PI3K-Akt pathway was the consistent pathway of functional enrichment analyses. Western blot assay showed that the protein levels in the prednisone + YQZY group had a tendency to the normal group. Finally, CASP3 and TNF were identified as the core targets that had strong binding to the active ingredients. YQZY might be a potential alternative approach in treating ITP through the PI3K-Akt pathway.
探讨益气滋阴方（YQZY）治疗免疫性血小板减少症（ITP）的作用机制及潜在靶点。对ITP小鼠口服泼尼松和YQZY。治疗后，采集血样评估血常规。取脾脏观察组织形态学变化并计算脾脏指数。基于中药系统药理学数据库（TCMSP）获取YQZY的活性成分和预测的相关靶点。通过GeneCards和DisGeNET数据库筛选ITP相关靶点。然后进行功能富集分析，以研究YQZY在ITP中的潜在调控途径，并通过蛋白质免疫印迹法进行验证。此外，构建了三种不同的网络，以确定YQZY在ITP治疗中的主要靶点和成分，随后进行分子对接分析。YQZY显著上调血小板计数，并改善了抗血小板血清注射引起的其他血液指标异常。共获得60种活性成分，以及活性成分与ITP的85个共同靶点。PI3K-Akt通路是功能富集分析中一致的通路。蛋白质免疫印迹法显示，泼尼松+YQZY组的蛋白水平有向正常组发展的趋势。最后，确定半胱天冬酶3（CASP3）和肿瘤坏死因子（TNF）为与活性成分有强结合的核心靶点。YQZY可能是通过PI3K-Akt通路治疗ITP的一种潜在替代方法。
