Lapina Tatiana, Statinov Vladislav, Vlasova Vitalina, Ermilova Elena
Biological Faculty, Saint-Petersburg State University, Saint-Petersburg, Russia.
Protoplasma. 2025 Aug 5. doi: 10.1007/s00709-025-02101-w.
Nitric oxide (NO) functions as a signaling molecule in many biological processes in green algae and higher plants. Although the mechanisms of NO synthesis in most plants are the subject of ongoing research and debate, a functional NO synthase (NOS) has been characterized only in Ostreococcus tauri. To date, the question of whether NO synthesis occurs in other NOS-containing members of the class Mamiellophyceaea, which gave rise to the core Chlorophyta, has not been elucidated. We found that, like O. tauri, O. lucimarinus and Bathycoccus prasinos grow on arginine as the sole nitrogen source, and their NOSs function and produce NO in cells. Moreover, in O. tauri, O. lucimarinus, and B. prasinos, NO exerts its biological functions through protein S-nitrosylation. Collectively, our data suggest that both NO and S-nitrosylated proteins are important mediators in the process of cell growth in NOS-containing representatives of Mamiellophyceae. Thus, we have updated the data related to protein S-nitrosylation as an evolutionarily conserved mechanism regulating many aspects of cell signaling in plants.
一氧化氮（NO）在绿藻和高等植物的许多生物过程中作为信号分子发挥作用。尽管大多数植物中NO合成的机制仍是正在进行研究和争论的主题，但仅在莱茵衣藻中鉴定出了一种功能性一氧化氮合酶（NOS）。迄今为止，在产生核心绿藻门的Mamiellophyceae类的其他含NOS成员中是否发生NO合成的问题尚未阐明。我们发现，与莱茵衣藻一样，明亮衣藻和原绿球藻以精氨酸作为唯一氮源生长，并且它们的NOS在细胞中发挥功能并产生NO。此外，在莱茵衣藻、明亮衣藻和原绿球藻中，NO通过蛋白质S-亚硝基化发挥其生物学功能。总体而言，我们的数据表明，NO和S-亚硝基化蛋白都是Mamiellophyceae含NOS代表中细胞生长过程中的重要介质。因此，我们更新了与蛋白质S-亚硝基化相关的数据，它是一种调节植物细胞信号传导多个方面的进化保守机制。
