Wang Yong, Guo Xu, Cui Yongqiu, Zhou Yu, Yang Kankan, Fu Ziteng, Sun Jianfei, Liu Guangqing, Cheng Bangzhao, Jiang Shudong, Li Yongdong
Anhui Province Key Laboratory of Veterinary Pathobiology and Disease Control, College of Animal Science and Technology, Anhui Agricultural University, Hefei, 230036 People's Republic of China.
Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai, 200241 People's Republic of China.
3 Biotech. 2020 Aug;10(8):354. doi: 10.1007/s13205-020-02308-z. Epub 2020 Jul 27.
This study aimed to explore the phylogenetic and molecular characteristics of feline astrovirus. A total of 33 fecal samples of domestic cats with or without diarrhea were collected from the Anhui province, and two positive samples were detected. The complete genome and of the two strains were sequenced and phylogenetically analyzed. AH-1-2020 and AH-2-2020 displayed 83.4% homology, and their homologies with other reference strains were 75.3%-83.4% and 83.4%-95.0%, respectively. Phylogenetic tree analysis revealed that all strains could be classified into three different clusters; therefore, the mean amino acid genetic distances (p-dist) among the three clusters were estimated. The results suggested that the two strains and other FeAstV strains were grouped into three genotypes, with AH-1-2020 belonging to a novel genotype. High similarity was observed (65.9%-66.5% nucleotide identity and 63.8%-64.8% amino acid identity) in ORF2 between porcine astrovirus type 1 and AH-1-2020. Furthermore, inter-specific recombination between porcine astrovirus type 1 and FeAstV was observed. We, therefore, inferred that inter-specific transmission may exist between pigs and cats; however, further studies are required to verify this. This is the first report on the genetic characterization and phylogenetic analysis of FeAstVs in the Anhui province and would further the current understanding of the genetic diversity and epidemiology of FeAstVs.
本研究旨在探讨猫星状病毒的系统发育和分子特征。从安徽省共采集了33份有或无腹泻症状的家猫粪便样本，检测到两份阳性样本。对这两个毒株的全基因组进行了测序和系统发育分析。AH-1-2020和AH-2-2020的同源性为83.4%，它们与其他参考毒株的同源性分别为75.3%-83.4%和83.4%-95.0%。系统发育树分析表明，所有毒株可分为三个不同的簇；因此，估计了这三个簇之间的平均氨基酸遗传距离（p距离）。结果表明，这两个毒株和其他猫星状病毒毒株被分为三个基因型，其中AH-1-2020属于一个新的基因型。1型猪星状病毒与AH-1-2020在开放阅读框2中具有较高的相似性（核苷酸同一性为65.9%-66.5%，氨基酸同一性为63.8%-64.8%）。此外，还观察到1型猪星状病毒与猫星状病毒之间的种间重组。因此，我们推断猪和猫之间可能存在种间传播；然而，需要进一步研究来证实这一点。这是安徽省关于猫星状病毒基因特征和系统发育分析的首次报道，将进一步加深目前对猫星状病毒遗传多样性和流行病学的了解。
