Moradi Ali, Nazarian Hamid, Shams Mofarahe Zahra, Kazemi Mahsa, Mosleh Hamidreza, Ghaffari Novin Mahsa, Kamran Ghomeshi Seyed, Chien Sufan, Bayat Mohammad, Ghaffari Novin Marefat
Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Statistics, University of Qom, Qom, Iran.
Lasers Med Sci. 2025 Aug 6;40(1):330. doi: 10.1007/s10103-025-04563-z.
Asthenozoospermia (AZS) affects approximately 20% of males. We aimed to determine the effectiveness of different photobiomodulation (PBM) protocols and naringenin (NAR) alone and in combination on sperm motility (SM) in AZS patients in vitro. We studied the different PBM protocols, including 0.6, 1.2, and 2.4 J/cm, by utilizing the RED (630 nm) and near-infrared (NIR) (810 nm) laser and different NAR concentrations (5, 10, 25, and 50 µmol/L), on sperms of AZS patients. In addition, the best PBM and NAR protocols were studied together. Evaluation methods included progressive sperm motility (PSM), sperm viability (SV), and DNA Fragmentation Index (DFI). The PSM results of RED and NIR showed a significant increase after 60 min compared with RED + NIR. NIR showed better results in PSM compared to other regimes (p < 0.02). The PSM data illustrated that 10 µmol/L of NAR had better results compared to other NAR groups (p < 0.01). The combination of optimal PBM and NAR regimes demonstrated an extraordinary promotion in PSM. NIR + NAR at 1.2 J/cm showed a better result in terms of PSM compared to other regimes (p < 0.01) and their DFI was decreased. A meaningful DFI increment was observed in the RED + NIR group compared to the other PBM groups (p < 0.05). DFI in 10 µmol/L of NAR was lesser than 50 µmol/L (p < 0.02). The combination of NIR (1.2 J/cm²) and NAR (10 µmol/L) significantly improved PSM and reduced DNA fragmentation, making it a promising approach for managing AZS.
弱精子症（AZS）影响约20%的男性。我们旨在确定不同的光生物调节（PBM）方案以及单独使用和联合使用柚皮素（NAR）对体外弱精子症患者精子活力（SM）的有效性。我们通过使用红色（630纳米）和近红外（NIR）（810纳米）激光以及不同浓度的NAR（5、10、25和50微摩尔/升），研究了不同的PBM方案，包括0.6、1.2和2.4焦耳/平方厘米，对弱精子症患者的精子。此外，还对最佳的PBM和NAR方案进行了联合研究。评估方法包括精子前向运动活力（PSM）、精子存活率（SV）和DNA碎片化指数（DFI）。红色和近红外光照射60分钟后的PSM结果与红色+近红外光照射相比有显著增加。与其他方案相比，近红外光在PSM方面显示出更好的结果（p<0.02）。PSM数据表明，10微摩尔/升的NAR与其他NAR组相比有更好的结果（p<0.01）。最佳的PBM和NAR方案联合使用在PSM方面有显著提升。1.2焦耳/平方厘米的近红外光+NAR在PSM方面比其他方案显示出更好的结果（p<0.01），并且其DFI降低。与其他PBM组相比，红色+近红外光组观察到DFI有显著增加（p<0.05）。10微摩尔/升的NAR中的DFI低于50微摩尔/升（p<0.02）。近红外光（1.2焦耳/平方厘米²）和NAR（10微摩尔/升）联合使用显著提高了PSM并减少了DNA碎片化，使其成为治疗弱精子症的一种有前景的方法。
