Malahom Nutthaporn, Boonthod Sukanya, Veerasup Nattapong, Pajarean Chaiwat, Prakobkij Akarapong, Anutrasakda Wipark, Citterio Daniel, Amatatongchai Maliwan, Nacapricha Duangjai, Jarujamrus Purim
Futuristic Science Research Center, School of Science, Walailak University, 222 Thaiburi, Thasala District, Nakhon Si Thammarat, 80160, Thailand.
Research Center for Theoretical Simulation and Applied Research in Bioscience and Sensing, Walailak University, Thasala, Nakhon Si Thammarat, 80160, Thailand.
Mikrochim Acta. 2025 Aug 6;192(9):561. doi: 10.1007/s00604-025-07412-6.
Two selective methods were developed for detecting mercury ions (Hg⁺) in food and water samples using catalytic fluorometry based on Au-Hg amalgamation with enhanced peroxidase-like activity. These approaches include (i) a laser-printed microfluidic paper-based analytical device (LP-µPAD) and (ii) a paper-based microcentrifuge tube test kit. The LP-µPAD was fabricated via commercial laser printing and integrated gold nanoparticles (AuNPs) with o-phenylenediamine (OPD) and hydrogen peroxide (H₂O₂). The test kit enabled Hg⁺ detection in food by reducing Hg⁺ to Hg⁰ with stannous chloride, facilitating Au-Hg amalgam formation in the detection zone. Both approaches utilized the Au-Hg amalgam to catalyze H₂O₂-mediated oxidation of OPD, generating fluorescent 2,3-diaminophenazine (DAP), further enhancing fluorescence intensity in proportion to Hg⁺ concentrations. The LP-µPAD exhibited a detection range of 3.0-20.0 µg L⁻ with a limit of detection (LOD) of 1.65 µg L⁻, whereas the test kit provided a detection range of 0.1-1.0 mg L⁻ with an LOD of 0.08 mg L⁻. Both sensors showed high selectivity for Hg⁺ over other ions and performed well in real sample analyses, aligning closely with results from conventional methods.
基于具有增强过氧化物酶样活性的金汞齐化反应，开发了两种利用催化荧光法检测食品和水样中汞离子（Hg⁺）的选择性方法。这些方法包括：（i）一种激光打印的基于微流控纸的分析装置（LP-µPAD）和（ii）一种基于纸的微量离心管测试试剂盒。LP-µPAD通过商业激光打印制造，并将金纳米颗粒（AuNPs）与邻苯二胺（OPD）和过氧化氢（H₂O₂）集成在一起。该测试试剂盒通过用氯化亚锡将Hg⁺还原为Hg⁰，促进检测区中金汞齐的形成，从而实现食品中Hg⁺的检测。两种方法都利用金汞齐催化H₂O₂介导的OPD氧化，生成荧光性的2,3-二氨基吩嗪（DAP），荧光强度进一步与Hg⁺浓度成比例增强。LP-µPAD的检测范围为3.0 - 20.0 μg L⁻，检测限（LOD）为1.65 μg L⁻，而测试试剂盒的检测范围为0.1 - 1.0 mg L⁻，LOD为0.08 mg L⁻。两种传感器对Hg⁺的选择性均高于其他离子，并且在实际样品分析中表现良好，与传统方法的结果非常吻合。
