从印度牛群中分离出猪布鲁氏菌：全基因组分析揭示人畜共患病传播的可能性。

Isolation of Brucella suis from cattle in India: whole genome analysis unravels potential for zoonotic transmission.

作者信息

Kumar M Suman, Vakamalla S S R, Ayoub Haris, Lalsangzuala C, Chandni A R, Dhanze Himani, Kunjukunju Vinod Valil, Anbazhagan S, Thomas Prasad

机构信息

Indian Veterinary Research Institute, Bareilly, India.

ICMR-National Animal Resource Facility for Biomedical Reserch, Hyderabad, India.

出版信息

Braz J Microbiol. 2025 Aug 6. doi: 10.1007/s42770-025-01747-1.

DOI:10.1007/s42770-025-01747-1

PMID:40768030

Abstract

Brucellosis is among the most widespread zoonotic diseases globally, affecting multiple domestic animal species. We report the first isolation of Brucella suis from a vaginal swab collected from an aborted cow in India. The isolate (VS1) was confirmed as B. suis bv. 1 by biochemical assays and species-specific PCR. Whole genome sequencing analysis of the VS1 isolate revealed a 32,81,903 bp genome with a guanosine and cytosine (GC) content of 57.29%. Multilocus sequence typing (MLST) 9-gene, 21-gene, and core genome MLST (cgMLST) schemes identified sequence types ST14, ST75, and ST1054/1075, respectively. Comparative genomics involving publicly available B. suis genome sequences showed conservation of key virulence genes. The genome B. suis strain 1330 was used to identify the single nucleotide polymorphisms (SNP) in study genomes and 63 SNPs were identified in the virulence-associated genes. The study indicates that B. suis biovar 1 can infect cattle, and likely contributes in the epidemiology and control of bovine brucellosis in India.

摘要

布鲁氏菌病是全球分布最广泛的人畜共患病之一，可感染多种家畜。我们报告了在印度一头流产奶牛的阴道拭子中首次分离出猪布鲁氏菌。通过生化分析和种特异性PCR确认该分离株（VS1）为猪布鲁氏菌生物变种1。对VS1分离株的全基因组测序分析显示，其基因组大小为3281903bp，鸟嘌呤和胞嘧啶（GC）含量为57.29%。多位点序列分型（MLST）的9基因、21基因和核心基因组MLST（cgMLST）方案分别鉴定出序列类型ST14、ST75和ST1054/1075。涉及公开可用的猪布鲁氏菌基因组序列的比较基因组学显示关键毒力基因具有保守性。使用猪布鲁氏菌菌株1330的基因组来鉴定研究基因组中的单核苷酸多态性（SNP），并在毒力相关基因中鉴定出63个SNP。该研究表明，猪布鲁氏菌生物变种1可感染牛，可能在印度牛布鲁氏菌病的流行病学和防控中发挥作用。