Wang Yuanyuan, Liu Yi, Yang Yexin, Bian Chao, Liu Chao, Zhang Yang, Mu Xidong
Key Laboratory of Prevention and Control for Aquatic Invasive Alien Species, Ministry of Agriculture and Rural Affairs, Guangdong Modern Recreational Fisheries Engineering Technology Center, Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.
Laboratory of Aquatic Genomics, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, 518060, China.
Sci Data. 2025 Aug 6;12(1):1367. doi: 10.1038/s41597-025-05720-3.
Electrophorus voltai, a South American electric eel, is renowned as the most powerful bioelectricity generator, capable of producing electric discharges reaching 860 volts. This remarkable ability positions it as an invaluable model for investigating the genetic, physiological, and developmental mechanisms driving electrogenesis in vertebrates. Here, we report a chromosome-level genome assembly of E. voltai, constructed using PacBio HiFi long reads and Hi-C scaffolding. The final assembly spans 666.91 Mb, with a contig N50 of 1.54 Mb and a scaffold N50 of 28.42 Mb, anchored onto 26 chromosomes. Genome annotation revealed 23,221 protein-coding genes, of which 22,306 genes were functionally annotated. Repetitive sequences account for 298.83 Mb of the genome, dominated by transposable elements. Additionally, we identified 225 miRNAs, 5,409 tRNAs, 288 rRNAs, and 535 snRNAs. This genome provides a foundational resource for comparative genomic studies of electric fish and facilitates investigations into the evolution of electrogenesis, electrocyte development, and bioelectric signal regulation in vertebrates.
伏打电鳗（Electrophorus voltai）是一种南美电鳗，被誉为最强大的生物电发生器，能够产生高达860伏的放电。这种非凡的能力使其成为研究脊椎动物电生成的遗传、生理和发育机制的宝贵模型。在此，我们报告了伏打电鳗的染色体水平基因组组装，该组装使用PacBio HiFi长读长和Hi-C支架构建。最终组装的基因组跨度为666.91 Mb，重叠群N50为1.54 Mb，支架N50为28.42 Mb，锚定在26条染色体上。基因组注释显示有23,221个蛋白质编码基因，其中22,306个基因有功能注释。重复序列占基因组的298.83 Mb，主要由转座元件组成。此外，我们鉴定出225个miRNA、5,409个tRNA、288个rRNA和535个snRNA。该基因组为电鱼的比较基因组研究提供了基础资源，并有助于研究脊椎动物电生成、电细胞发育和生物电信号调节的进化。
