Higuchi A
Hokkaido Igaku Zasshi. 1985 Sep;60(5):748-62.
An abnormal fibrinogen in patients with liver diseases, especially liver cirrhosis and hepatocellular carcinoma was examined. In these patients, delayed polymerization of fibrin monomer, which was useful for detecting abnormal fibrinogen in plasma and also detecting one of liver dysfunctions, was observed. Same results were found by using purified abnormal fibrinogen from these patients. However, according to electrophoretic and immunochemical studies, no difference were shown between purified abnormal fibrinogen and purified normal fibrinogen. The total content of sialic acid in purified abnormal fibrinogen was markedly increased as compared to that in purified normal fibrinogen. When coagulation time was examined by using asialofibrinogen treated with neuraminidase, the prolonged coagulation time was partially normalized even in patients with liver cirrhosis. These findings suggested that sialic acid might affect the polymerization of fibrin monomer. It was reported by Harvey (1978) that an abnormal fibrinogen in liver diseases was similar to the fetal fibrinogen in the content of sialic acid and prolongation of thrombin time. Therefore, purified fibrinogen from umbilical cord blood was also investigated by similar methods. Consequently, it was suggested that a dysfunction of fibrinogen in umbilical cord blood was not related to molecular abnormality, but some inhibitory mechanisms which caused the abnormal pattern of coagulation might be existed.
对患有肝脏疾病，尤其是肝硬化和肝细胞癌患者的异常纤维蛋白原进行了检测。在这些患者中，观察到纤维蛋白单体延迟聚合，这有助于检测血浆中的异常纤维蛋白原以及检测肝功能障碍之一。使用从这些患者中纯化的异常纤维蛋白原也得到了相同的结果。然而，根据电泳和免疫化学研究，纯化的异常纤维蛋白原与纯化的正常纤维蛋白原之间未显示出差异。与纯化的正常纤维蛋白原相比，纯化的异常纤维蛋白原中唾液酸的总含量明显增加。当使用经神经氨酸酶处理的去唾液酸纤维蛋白原检测凝血时间时，即使在肝硬化患者中，延长的凝血时间也部分恢复正常。这些发现表明唾液酸可能影响纤维蛋白单体的聚合。哈维（1978年）报道，肝脏疾病中的异常纤维蛋白原在唾液酸含量和凝血酶时间延长方面与胎儿纤维蛋白原相似。因此，也通过类似方法对脐带血中的纯化纤维蛋白原进行了研究。结果表明，脐带血中纤维蛋白原的功能障碍与分子异常无关，但可能存在一些导致异常凝血模式的抑制机制。
