Ito M, Karmali R, Krim M
Immunology. 1985 Nov;56(3):533-41.
Considerably augmented chemiluminescence (CL) occurred when murine peritoneal resident macrophages (MPs), pretreated with murine interferon (MuIFN)-alpha within 24 hr, were stimulated by 4-beta-phorbol, 12-beta-myristate, 13-beta-acetate (PMA). Augmentation of CL generation ceased when incubation in the presence of MuIFN was continued for 48 hr. As 12 hr preincubation with MuIFN procured optimal CL generation, the various reactive oxygen species (OH, O2-., H2O2) were measured at that point. The hydroxyl radical (OH.) level in MuIFN-treated MPs was 19.44 times higher than in MuIFN-untreated MPs. However, the levels of O2-. and H2O2 generation were the same in both MuIFN-treated and untreated MPs. Moreover, by using the inhibitors lipoxygenase and cyclo-oxygenase, we established clearly that CL and OH. generation in MuIFN-treated MPs is due to the lipoxygenase pathway of arachidonic acid metabolism.
当在24小时内用鼠干扰素(MuIFN)-α预处理的小鼠腹腔驻留巨噬细胞(MPs)受到4-β-佛波醇、12-β-肉豆蔻酸酯、13-β-乙酸酯(PMA)刺激时,化学发光(CL)显著增强。当在MuIFN存在下继续孵育48小时时,CL生成的增强停止。由于用MuIFN预孵育12小时可获得最佳的CL生成,因此在该时间点测量了各种活性氧(OH、O2-·、H2O2)。经MuIFN处理的MPs中的羟基自由基(OH·)水平比未用MuIFN处理的MPs高19.44倍。然而,经MuIFN处理和未处理的MPs中O2-·和H2O2的生成水平相同。此外,通过使用脂氧合酶和环氧化酶抑制剂,我们明确确定经MuIFN处理的MPs中的CL和OH·生成是由于花生四烯酸代谢的脂氧合酶途径。