Boraschi D, Ghezzi P, Pasqualetto E, Salmona M, Nencioni L, Soldateschi D, Villa L, Tagliabue A
Immunology. 1983 Nov;50(3):359-68.
Mouse peritoneal macrophages (M phi) expressed enhanced tumoricidal activity upon in vitro stimulation either with the lymphokine M phi-activating factor (MAF) or with fibroblast interferon (IFN-beta). In contrast, M phi suppressive activity on lymphoproliferation was not affected by MAF pretreatment, but was drastically reduced or abolished by IFN-beta. Catalase, the enzyme involved in the destruction of hydrogen peroxide (H2O2), did significantly decrease M phi suppressive capacity but had no effect on M phi tumoricidal activity. Analysis of the phagocytosis-dependent H2O2 production by IFN-beta-treated M phi demonstrated a strong impairment of the oxygen metabolite release, which strictly paralleled the decreased M phi suppressive capacity. On the other hand, MAF did not modify H2O2 release by M phi. Studies on M phi antibacterial activity against Salmonella typhimurium, a function thought to depend upon H2O2 production, showed that exposure of M phi to IFN-beta significantly impaired their bactericidal and bacteriostatic capacity, again in close correlation with the decrease in H2O2 production. Thus, IFN-beta appears as modulating both suppressive and antibacterial capacities of M phi through reduction of their oxygen metabolism, whereas regulation of M phi anti-tumour activity is possibly controlled by different mechanisms.
小鼠腹腔巨噬细胞（M phi）在体外受到淋巴因子巨噬细胞激活因子（MAF）或成纤维细胞干扰素（IFN-β）刺激后，表现出增强的杀瘤活性。相比之下，MAF预处理对M phi对淋巴细胞增殖的抑制活性没有影响，但IFN-β可使其显著降低或消除。过氧化氢酶参与过氧化氢（H2O2）的破坏，它确实显著降低了M phi的抑制能力，但对M phi的杀瘤活性没有影响。对经IFN-β处理的M phi吞噬依赖性H2O2产生的分析表明，氧代谢产物的释放受到严重损害，这与M phi抑制能力的降低密切相关。另一方面，MAF不会改变M phi释放H2O2的情况。对M phi抗鼠伤寒沙门氏菌抗菌活性的研究表明，M phi暴露于IFN-β会显著损害其杀菌和抑菌能力，这再次与H2O2产生的减少密切相关。因此，IFN-β似乎通过降低M phi的氧代谢来调节其抑制和抗菌能力，而M phi抗肿瘤活性的调节可能由不同机制控制。
