Schneider C H, Guenin R
Int J Immunopharmacol. 1985;7(6):933-7. doi: 10.1016/0192-0561(85)90057-8.
N1-DNCP-N6-lactobionoyl-1,6,hexanediamine, which is a potent anaphylactogen when i.v. injected into anti-DNCP sensitized guinea pigs, was shown to be also anaphylactogenic when administered intradermally into guinea pigs passively sensitized by i.v. injection of anti-DNCP antiserum. The compound, which is monohaptenic conjugate with the DNCP group as haptenic part and the carbohydrate moiety as auxiliary group, was found to be unable to precipitate high-titered anti-DNCP antisera. This precludes formation of simple, functionally oligovalent associates. Since many monohaptenic anaphylactogens with various hydrocarbon chains as auxiliary groups do not induce anaphylaxis when given intradermally, the anaphylaxis, demonstrated after intradermal antigen application, allows a biological distinction between the lactobionoyl and similar conjugates, and the hydrocarbon-bearing anaphylactogens.
N1 - 二硝基氯苯 - N6 - 乳糖二酰基 - 1,6 - 己二胺，静脉注射到抗二硝基氯苯致敏的豚鼠体内时是一种强效过敏原，经皮内注射到经静脉注射抗二硝基氯苯抗血清被动致敏的豚鼠体内时也显示出过敏反应。该化合物是一种单半抗原共轭物，其半抗原部分为二硝基氯苯基团，辅助基团为碳水化合物部分，发现它不能沉淀高滴度的抗二硝基氯苯抗血清。这排除了形成简单的、功能上多价缔合物的可能性。由于许多以各种烃链作为辅助基团的单半抗原过敏原经皮内注射时不会诱发过敏反应，因此在皮内应用抗原后表现出的过敏反应，使得乳糖二酰基共轭物及类似共轭物与含烃类过敏原之间在生物学上得以区分。
