Rahman Mahbubur, Kabir Mohashin, Liu Hailei, Zhang Li, Chen Shaojuan, Wu Shaohua
College of Textiles and Clothing, Qingdao University, Qingdao 266071, China; Department of Textile Engineering, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh.
College of Textiles and Clothing, Qingdao University, Qingdao 266071, China.
Biomater Adv. 2026 Jan;178:214441. doi: 10.1016/j.bioadv.2025.214441. Epub 2025 Aug 6.
The development and design of novel nanofibrous dressings (NFDs) with biomimetic structure and multifunctional properties through electrospinning technique have aroused significant interest in the disciplines of wound treatment. In this study, curcumin (Cur) was firstly incorporated into zeolitic imidazolate framework-8 (ZIF-8), to generate Cur@ZIF-8 nanoparticles, which displayed a homogeneous, consistent, and nearly similar rhombic dodecahedral structure with the mean diameter of 190 nm. Then the Cur@ZIF-8 was loaded into the silk fibroin (SF)/polycaprolactone (PCL) hybrid nanofibers to obtain a NFD through an electrospinning strategy. The other three different NFDs, i.e., SF/PCL, SF/PCL/ZIF-8, and SF/PCL/Cur, were fabricated as control groups. All the four different NFDs were found to exhibit uniform and bead-free morphology. Among them, the SF/PCL/Cur@ZIF-8 NFD exhibited superior mechanical properties, high porosity (83.8 ± 3.7 %), and water uptake (292.4 ± 9.5 %). Importantly, the SF/PCL/Cur@ZIF-8 NFD showed a controlled Cur release profile, fitting the Krosmeyer-Peppas model. The in vitro cell experiments indicated that the SF/PCL/Cur@ZIF-8 NFD obviously improved the proliferation and adhesion of human dermal fibroblasts (HDFs). Moreover, the SF/PCL/Cur@ZIF-8 NDF also exhibited great antibacterial activity, with the inhibition rate of 83.2 ± 1.7 % and 80.2 ± 4.7 % against E. coli and S. aureus, correspondingly. In vivo, the SF/PCL/Cur@ZIF-8 NFD not only significantly reduced the bleeding amount in a mouse liver hemorrhage model, but also obviously accelerated the wound healing (99 %) by augmenting the collagen growth and re-epithelialization. The present study revealed that the SF/PCL/Cur@ZIF-8 NFD generated by a combination of metal-organic framework (MOF) synthesis and electrospinning showed significant promise for applications in wound treatment and skin tissue engineering.
通过静电纺丝技术开发和设计具有仿生结构和多功能特性的新型纳米纤维敷料（NFD），已引起伤口治疗领域的广泛关注。在本研究中，首先将姜黄素（Cur）掺入沸石咪唑酯骨架-8（ZIF-8）中，生成Cur@ZIF-8纳米颗粒，其呈现出均匀、一致且近乎相似的菱形十二面体结构，平均直径约为190nm。然后通过静电纺丝策略将Cur@ZIF-8负载到丝素蛋白（SF）/聚己内酯（PCL）混合纳米纤维中，以获得一种NFD。另外制备了三种不同的NFD，即SF/PCL、SF/PCL/ZIF-8和SF/PCL/Cur作为对照组。发现所有四种不同的NFD均呈现出均匀且无珠的形态。其中，SF/PCL/Cur@ZIF-8 NFD表现出优异的机械性能、高孔隙率（83.8±3.7%）和吸水率（292.4±9.5%）。重要的是，SF/PCL/Cur@ZIF-8 NFD显示出可控的Cur释放曲线，符合Krosmeyer-Peppas模型。体外细胞实验表明，SF/PCL/Cur@ZIF-8 NFD明显促进了人皮肤成纤维细胞（HDF）的增殖和黏附。此外，SF/PCL/Cur@ZIF-8 NDF还表现出很强的抗菌活性，对大肠杆菌和金黄色葡萄球菌的抑制率分别为83.2±1.7%和80.2±4.7%。在体内，SF/PCL/Cur@ZIF-8 NFD不仅在小鼠肝出血模型中显著减少了出血量，还通过促进胶原蛋白生长和再上皮化明显加速了伤口愈合（约99%）。本研究表明，通过金属有机框架（MOF）合成与静电纺丝相结合产生的SF/PCL/Cur@ZIF-8 NFD在伤口治疗和皮肤组织工程应用中显示出巨大的潜力。
