Fabiszewska Agata, Piasecka-Jóźwiak Katarzyna, Choińska Renata, Wróbel Barbara, Miecznikowski Antoni, Wierzchowska Katarzyna, Świętochowski Adam, Dąbrowska Magdalena, Bujak Marzena, Reshetiuk Volodymyr, Lisowski Aleksander
Department of Chemistry, Institute of Food Sciences, Warsaw University of Life Sciences, 159 c Nowoursynowska Street, 02-787 Warsaw, Poland.
Department of Fermentation Technology, Prof. Wacław Dąbrowski Institute of Agricultural and Food Biotechnology-State Research Institute, Rakowiecka 36 Street, 02-532 Warsaw, Poland.
Bioresour Technol. 2025 Dec;437:133107. doi: 10.1016/j.biortech.2025.133107. Epub 2025 Aug 5.
This study investigated the effect of co-fermentation by Pediococcus acidilactici KKP 2065p and Lentilactobacillus buchneri KKP 2047p on propionic acid production and biogas yield from grass silage. The research was conducted in three stages. First, the growth dynamics and acid production of mono- and co-cultures were assessed in modified MRS media supplemented with glucose and 1,2-propanediol (1,2-PDO) under laboratory conditions. Len. buchneri monoculture produced the highest concentration of propionic acid (3.06 g/L), while co-culture with P. acidilactici yielded 2.88 g/L. In co-cultures of P. acidilactici KKP 2065p and Len. buchneri KKP 2047p, no synergistic increase in propionic acid or 1-propanol production was observed compared to monocultures. In MRS medium with 1,2-PDO, Len. buchneri primarily contributed to acetic acid synthesis, while P. acidilactici was responsible for propionic acid and propanol formation. Notably, a high concentration of 1-propanol was associated with a possible metabolic shift or intermediate accumulation. Second, silages were prepared from grass and inoculated with the selected LAB strains. Silage fermentation was evaluated through analysis, which revealed increased lactic acid content (7.30 vs. 2.83 g/kg DM in control) and a dominant presence of Lactobacillus (91.8 %) in inoculated silages, alongside a marked reduction in Weissella and Enterococcus spp. The synergism of the selected two strains significantly affected the increase in acetic acid content, which occurred more relevant in anaerobic digestion and biomethane yield in the third step when the biogas potential of the silages was assessed. Inoculated silages showed a 14-21 % increase in methane yield (up to 376 mL/g VS), with methane content reaching 46-55 % (compared to 36-45 % in the control). These results demonstrate that the combined use of P. acidilactici and L. buchneri can enhance both the volatile organic acids content in ensiled plant material and renewable energy output.
本研究调查了嗜酸乳杆菌KKP 2065p和布氏扁豆乳杆菌KKP 2047p共同发酵对青草青贮饲料丙酸产量和沼气产量的影响。该研究分三个阶段进行。首先，在实验室条件下，在添加葡萄糖和1,2 - 丙二醇（1,2 - PDO）的改良MRS培养基中评估单一培养物和混合培养物的生长动态及产酸情况。布氏扁豆乳杆菌单一培养物产生的丙酸浓度最高（3.06 g/L），而与嗜酸乳杆菌的混合培养物产生的丙酸浓度为2.88 g/L。在嗜酸乳杆菌KKP 2065p和布氏扁豆乳杆菌KKP 2047p的混合培养物中，与单一培养物相比，未观察到丙酸或1 - 丙醇产量的协同增加。在含有1,2 - PDO的MRS培养基中，布氏扁豆乳杆菌主要促进乙酸合成，而嗜酸乳杆菌负责丙酸和丙醇的形成。值得注意的是，高浓度的1 - 丙醇可能与代谢转变或中间产物积累有关。其次，用青草制备青贮饲料，并接种所选的乳酸菌菌株。通过分析评估青贮饲料发酵情况，结果显示接种青贮饲料中乳酸含量增加（对照为2.83 g/kg DM，接种后为7.30 g/kg DM），且乳酸菌占主导地位（91.8%），同时魏斯氏菌属和肠球菌属显著减少。所选两种菌株的协同作用显著影响了乙酸含量的增加，在第三步评估青贮饲料的沼气潜力时，这种增加在厌氧消化和生物甲烷产量方面更为明显。接种青贮饲料的甲烷产量增加了14 - 21%（高达376 mL/g VS），甲烷含量达到46 - 55%（对照为36 - 45%）。这些结果表明，嗜酸乳杆菌和布氏扁豆乳杆菌联合使用可提高青贮植物材料中的挥发性有机酸含量以及可再生能源产量。
