Peñaherrera Eugenia, Sarmiento-Pacurucu Josue, Santos-Ordóñez Efrén, Kachatryan Alexandr, Cuzco Nancy, Vanegas David, Calle-López Jessica, Villao-Uzho Liliana, Heyden Yvan Vander, Wilches Isabel, León-Tamariz Fabián
Department of Biosciences, Group of Medicinal Plants and Natural Products, Faculty of Chemistry, Universidad de Cuenca, Cuenca, Ecuador.
Faculty of Life Sciences, ESPOL Polytechnic University, Escuela Superior Politécnica del Litoral, ESPOL, Guayaquil, Ecuador.
Front Plant Sci. 2025 Jul 24;16:1612556. doi: 10.3389/fpls.2025.1612556. eCollection 2025.
In the Ecuadorian traditional medicine, two species of the Desmodium genus, and , are used interchangeably for the treatment of various ailments, particularly those related to inflammatory processes, wound healing, stomach ulcers and liver disorders. Despite the extensive knowledge and characterization of , there is limited information regarding . This highlights the necessity for the development of analytical tools that facilitate the differentiation between these two species and the characterization of the latter. The tools were developed and evaluated at two distinct levels: genetically, using the DNA barcoding technique, and analytically, using chromatographic fingerprinting. Additionally, the antioxidant potential of the samples was evaluated through the establishment of the RACI index, based on various evaluation techniques. genetic DNA barcodes were obtained for and the phylogenetic analysis separated them from those obtained from , demonstrating that the , , and markers are the most effective for differentiating between the species. Additionally, the antioxidant potential of was found to be higher than that of . The apigenin 8-C-glucoside (vitexin), together with tannic and chlorogenic acids have been pointed by HPLC fingerprinting analysis as responsible for this pharmacological activity.
在厄瓜多尔传统医学中，山蚂蝗属的两个物种，即[物种名称1]和[物种名称2]，可互换用于治疗各种疾病，特别是与炎症过程、伤口愈合、胃溃疡和肝脏疾病相关的疾病。尽管对[物种名称1]已有广泛的了解和特征描述，但关于[物种名称2]的信息有限。这凸显了开发有助于区分这两个物种并对后者进行特征描述的分析工具的必要性。这些工具在两个不同层面进行了开发和评估：在基因层面，使用DNA条形码技术；在分析层面，使用色谱指纹图谱。此外，基于各种评估技术，通过建立RACI指数来评估样品的抗氧化潜力。获得了[物种名称1]和[物种名称2]的遗传DNA条形码，系统发育分析将它们与从[另一物种名称]获得的条形码区分开来，表明[具体标记名称1]、[具体标记名称2]和[具体标记名称3]标记对于区分这些物种最有效。此外，发现[物种名称2]的抗氧化潜力高于[物种名称1]。通过HPLC指纹图谱分析指出，芹菜素8 - C - 葡萄糖苷（牡荆素）以及鞣酸和绿原酸是这种药理活性的原因。
