Lee Kyung Jun, Baek Da-Young, Lee Gi-An, Cho Gyu-Taek, So Yoon-Sup, Lee Jung-Ro, Ma Kyung-Ho, Chung Jong-Wook, Hyun Do Yoon
National Agrobiodiversity Center, National Institute of Agricultural Sciences (NAS), RDA, Jeonju 54874, Korea.
Department of Crop Science, Chungbuk National University, Chungdae-ro 1, Seowon-Gu, Cheongju, Chungbuk 28644, Korea.
Antioxidants (Basel). 2020 Mar 5;9(3):213. doi: 10.3390/antiox9030213.
Black soybean ( L.) has been used as a traditional medicine because its seed coat contains various natural phenolic compounds such as anthocyanins. The objective of this study was to reveal the genetic variation in the agricultural traits, phytochemicals, and antioxidant activity of 172 Korean black soybean landraces (KBSLs) and establish a relationship among them. The evaluation of three agricultural traits (days to 50% flowering, maturity, and 100-seed weight), six phytochemicals (delphinidin-3-glucoside, cyaniding-3-glucoside, petunidin-3-glucoside, daidzin, glycitin, and genestin), and four antioxidant activities (2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS), ferric-reducing antioxidant power (FRAP), and the total polyphenol content (TPC) of 172 KBSLs were analyzed in 2012 and 2015. The agricultural traits, phytochemicals, and antioxidant activities of the 172 KBSLs showed wide variation among the accessions and years. In correlation analysis, the agricultural traits and phytochemicals showed positive and negative correlations with phytochemicals and antioxidant activity, respectively. The principal component analyses result indicated that phytochemicals accounted for most of the variability in the KBSLs. In clustering analysis, the 172 KBSLs were classified into four clusters. These results could lead to expanding the knowledge of the agricultural traits, phytochemicals, and antioxidant activity of the KBSLs, which are valuable materials for the development of new soybean varieties.
黑豆（Glycine max (L.) Merr.）一直被用作传统药物，因为其种皮含有多种天然酚类化合物，如花色苷。本研究的目的是揭示172个韩国黑豆地方品种（KBSLs）在农艺性状、植物化学物质和抗氧化活性方面的遗传变异，并建立它们之间的关系。在2012年和2015年对172个KBSLs的三个农艺性状（50%开花天数、成熟天数和百粒重）、六种植物化学物质（飞燕草素-3-葡萄糖苷、矢车菊素-3-葡萄糖苷、矮牵牛素-3-葡萄糖苷、大豆苷、甘草苷和染料木苷）和四种抗氧化活性（2,2-二苯基-1-苦基肼（DPPH）、2,2'-联氮-双-（3-乙基苯并噻唑啉-6-磺酸）（ABTS）、铁还原抗氧化能力（FRAP）和总多酚含量（TPC））进行了分析。172个KBSLs的农艺性状、植物化学物质和抗氧化活性在不同种质和年份间表现出广泛的变异。在相关性分析中，农艺性状与植物化学物质分别与植物化学物质和抗氧化活性呈正相关和负相关。主成分分析结果表明，植物化学物质占KBSLs变异的大部分。在聚类分析中，172个KBSLs被分为四个聚类。这些结果有助于扩展对KBSLs农艺性状、植物化学物质和抗氧化活性的认识，KBSLs是培育新大豆品种的宝贵材料。
