用于测定血液、尿液和胆汁中生物活性5-氟尿嘧啶的琼脂平板技术的方法学方面。

Methodological aspects of an agar plate technique for determination of biologically active 5-fluorouracil in blood, urine and bile.

作者信息

Brandberg A, Almersjö O, Falsen E, Gustavsson B, Hafström L, Lindblom G B

出版信息

Acta Pathol Microbiol Scand B. 1977 Jun;85(3):227-34. doi: 10.1111/j.1699-0463.1977.tb01700.x.

DOI:10.1111/j.1699-0463.1977.tb01700.x

Abstract

A microbiological agar plate technique for estimation of 5-fluorouracil concentrations in blood, urine and bile from man, dog and pig was evaluated. Different bacterial test strains, media modifications and techniques for inoculation were studied. The strain Streptococcus faecalis ATCC 8043, recommended previously by Clarkson et al., was found to be the most suitable. The influence of prediffusion, dilution, antibiotics and chemotherapeutic agents and their antagonists, as well as the effect of storage of samples containing 5-fluorouracil were examined. A detailed methodological description is presented. The method seems to be sufficiently sensitive and practical for routine determination of cytotoxic compounds from 5-fluorouracil in serum, plasma and urine.

摘要

对一种用于估算人、狗和猪的血液、尿液及胆汁中5-氟尿嘧啶浓度的微生物琼脂平板技术进行了评估。研究了不同的细菌测试菌株、培养基改良方法及接种技术。发现先前由克拉克森等人推荐的粪肠球菌ATCC 8043菌株最为合适。研究了预扩散、稀释、抗生素、化疗药物及其拮抗剂的影响，以及含5-氟尿嘧啶样品的储存效果。给出了详细的方法描述。该方法对于常规测定血清、血浆及尿液中5-氟尿嘧啶的细胞毒性化合物似乎足够灵敏且实用。