Hase S, Kikuchi N, Ikenaka T, Inoue K
J Biochem. 1985 Oct;98(4):863-74. doi: 10.1093/oxfordjournals.jbchem.a135366.
Human C3, the third component of human complement, contained mannose and N-acetylglucosamine as sugar components. The sugar chains were liberated from the polypeptide chains by hydrazinolysis, and the free amino groups were N-acetylated. The reducing end residues of the sugar chains thus obtained were tagged with 2-aminopyridine, and the pyridylamino (PA-) derivatives of sugar chains were separated by high-performance liquid chromatography. The structures of purified PA-sugar chains were analyzed by a combination of stepwise exoglycosidase digestions, size determination by paper electrophoresis, methylation analysis, Smith degradation, and partial acetolysis. These results showed that C3 contained two high-mannose type sugar chains ranging from Man5GlcNAc2 to Man9GlcNAc2. Analyses of the sugar chains of alpha- and beta-chains of C3 indicated that the alpha-chain contained mainly Man8GlcNAc2 and Man9GlcNAc2, while the beta-chain contained mainly Man5GlcNAc2 and Man6GlcNAc2.
人补体第三成分C3含有甘露糖和N - 乙酰葡糖胺作为糖成分。通过肼解从多肽链上释放糖链，并将游离氨基进行N - 乙酰化。将由此获得的糖链的还原端残基用2 - 氨基吡啶标记，糖链的吡啶基氨基（PA -）衍生物通过高效液相色谱法分离。通过逐步外切糖苷酶消化、纸电泳测定大小、甲基化分析、史密斯降解和部分乙酰解相结合的方法分析纯化的PA - 糖链的结构。这些结果表明C3含有两条高甘露糖型糖链，范围从Man5GlcNAc2到Man9GlcNAc2。对C3的α链和β链糖链的分析表明，α链主要含有Man8GlcNAc2和Man9GlcNAc2，而β链主要含有Man5GlcNAc2和Man6GlcNAc2。
