Golden M, Waterlow J C, Picou D
Am J Clin Nutr. 1977 Aug;30(8):1345-8. doi: 10.1093/ajcn/30.8.1345.
We have examined the relationships between protein turnover, protein synthesis, and protein breakdown and dietary intake, weight change, and nitrogen balance in children who were recovering and had recovered from severe protein-energy-malnutrition. Protein metabolism was measured by giving [15N]glycine and measuring the enrichment of urinary urea. The level of dietary protein did not affect protein metabolism. There were highly significant correlations between both protein flux and protein synthesis and the ad libitum dietary intake, nitrogen balance, and weight change. Over the range of dietary intake, 60 to 270 cal/kg per day, the protein synthesis rate increased 5-fold. Large changes in dietary intake resulted in small changes in protein breakdown, with breakdown being least on an inadequate intake. Changes in the rate of protein breakdown did not contribute to changes in nitrogen balance or body weight.
我们研究了严重蛋白质-能量营养不良恢复期及已康复儿童的蛋白质周转、蛋白质合成、蛋白质分解与饮食摄入、体重变化及氮平衡之间的关系。通过给予[15N]甘氨酸并测量尿尿素的富集量来测定蛋白质代谢。饮食蛋白质水平不影响蛋白质代谢。蛋白质通量和蛋白质合成与随意饮食摄入、氮平衡及体重变化之间均存在高度显著的相关性。在每天60至270千卡/千克的饮食摄入范围内，蛋白质合成率增加了5倍。饮食摄入量的大幅变化导致蛋白质分解的小幅变化，摄入量不足时分解最少。蛋白质分解率的变化对氮平衡或体重变化没有贡献。
