鸡和兔抗体在用于病毒检测的固相蛋白A放射免疫测定中的应用。

Use of chicken and rabbit antibodies in a solid phase protein A radioimmunossay for virus detection.

作者信息

Katz D, Lehrer S, Kohn A

出版信息

J Virol Methods. 1985 Oct;12(1-2):59-70. doi: 10.1016/0166-0934(85)90008-4.

Abstract

A new rapid indirect solid phase radioimmunoassay was developed for the detection of Sindbis virus. Chicken antibodies were adsorbed onto wells in microplates to serve as 'capture antibodies' and rabbit antibodies were used as the second antibody. 125I-labelled protein A that does not bind to chicken antibodies, but binds firmly to rabbit antibodies was used as the tracer. All the steps necessary for the development of the assay are described. The minimal amount of Sindbis virus detectable was around 3 x 10(5) PFU/ml and the interassay reproducibility was about +/- 30%.

摘要

一种用于检测辛德毕斯病毒的新型快速间接固相放射免疫测定法被开发出来。鸡抗体吸附在微孔板的孔中作为“捕获抗体”，兔抗体用作第二抗体。不与鸡抗体结合但能牢固结合兔抗体的125I标记蛋白A用作示踪剂。描述了该测定法开发所需的所有步骤。可检测到的辛德毕斯病毒的最小量约为3×10(5) PFU/ml，测定法之间的重现性约为±30%。