使用¹²⁵I标记的蛋白A检测对粗制鼠白血病病毒的体液免疫。

The use of 125I-labelled protein A for the detection of humoral immunity to gross murine leukaemia virus.

作者信息

Holmes H C, Tuffrey M, Steuden J, Hilgers J, Barnes R D

出版信息

J Immunol Methods. 1980;36(3-4):235-41. doi: 10.1016/0022-1759(80)90129-5.

Abstract

A solid-phase radioimmunoassay utilising bind of 125I-labelled protein A to antibodies bound to virus adsorbed onto microtitre plates was shown to be suitable for detection of humoral immunity to Gross murine leukaemia virus (MuLV). The specificity of the reaction was shown by the fact that only homologous or closely related viruses effectively inhibited binding of antibodies to adsorbed virus. With this method a low level of spontaneous humoral immunity was demonstrated in sera from AKR/Crc mice, a strain with high concentrations of endogenous virus, whereas little or no anti-viral activity was found inCBA/H-T6Crc, a subline that does not appear to express MuLV.

摘要

利用125I标记的蛋白A与吸附在微量滴定板上的病毒所结合的抗体相结合的固相放射免疫测定法，已证明适用于检测对格罗斯小鼠白血病病毒（MuLV）的体液免疫。该反应的特异性表现在只有同源或密切相关的病毒才能有效抑制抗体与吸附病毒的结合这一事实上。用这种方法在AKR/Crc小鼠（一种内源性病毒浓度高的品系）的血清中证明存在低水平的自发体液免疫，而在似乎不表达MuLV的CBA/H-T6Crc亚系中几乎没有发现抗病毒活性。