用于快速诊断辛德毕斯病毒抗原的固相间接放射免疫测定法。
Solid phase indirect radioimmunoassays for the rapid diagnosis of Sindbis virus antigen.
作者信息
Enzmann P J
出版信息
J Gen Virol. 1978 Dec;41(3):641-4. doi: 10.1099/0022-1317-41-3-641.
PMID:84855
Abstract
Indirect radioimmunoassays have been developed for the rapid detection of Sindbis virus. Dilutions of Sindbis virus from tissue culture fluids have been immobilized and allowed to react with rabbit anti-Sindbis virus antibodies. The bound antibodies were assayed either by 125I-labelled anti-rabbit IgG-antibodies or alternatively by addition of human complement and 125I-labelled anti-human C1q antibodies or 125I-labelled protein A.
摘要
已开发出间接放射免疫测定法用于快速检测辛德毕斯病毒。将来自组织培养液的辛德毕斯病毒稀释液固定化，并使其与兔抗辛德毕斯病毒抗体反应。结合的抗体通过125I标记的抗兔IgG抗体进行检测，或者通过添加人补体以及125I标记的抗人C1q抗体或125I标记的蛋白A进行检测。
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