抗T-2毒素单克隆抗体。体外中和蛋白质合成抑制作用及保护大鼠免受致死性毒血症侵害。
Monoclonal antibodies to T-2 toxin. In vitro neutralization of protein synthesis inhibition and protection of rats against lethal toxemia.
作者信息
Feuerstein G, Powell J A, Knower A T, Hunter K W
出版信息
J Clin Invest. 1985 Dec;76(6):2134-8. doi: 10.1172/JCI112218.
A murine monoclonal antibody (15H6) against the trichothecene mycotoxin T-2 was capable of neutralizing the in vitro protein synthesis inhibitory effect of T-2 toxin in human B lymphoblastoid cultures. It was further shown that 15H6 given to rats (250 mg/kg) 30 min before or 15 min after a lethal dose (1 mg/kg) of T-2 toxin conferred 100% survival. A lower dose of 15H6 (125 mg/kg), given 15 min after the lethal dose of T-2 toxin, protected 25% of the rats. An increased time to death and 45% survival was seen in rats given the full dose of 15H6 antibody 60 min after lethal toxin. These data are the first demonstration of effective prophylaxis and therapy for T-2 toxemia.
一种针对单端孢霉烯族霉菌毒素T-2的鼠源单克隆抗体（15H6）能够在体外人B淋巴母细胞培养物中中和T-2毒素对蛋白质合成的抑制作用。进一步研究表明，在给予致死剂量（1 mg/kg）的T-2毒素前30分钟或后15分钟给大鼠注射15H6（250 mg/kg），可使大鼠100%存活。在给予致死剂量的T-2毒素后15分钟给予较低剂量的15H6（125 mg/kg），可保护25%的大鼠。在给予致死毒素60分钟后给予全剂量15H6抗体的大鼠中，观察到死亡时间延长，存活率为45%。这些数据首次证明了对T-2毒血症有效的预防和治疗作用。
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