Bishop J R, Bodine A B, O'Dell G D, Janzen J J
J Dairy Sci. 1985 Nov;68(11):3031-6. doi: 10.3168/jds.S0022-0302(85)81198-X.
Delvotest-P and Bacillus stearothermophilus Difco disc assay procedures were utilized, and assay sensitivity for detecting various antibiotics was tested. Bacillus stearothermophilus spores used in the disc assay were purchased from two laboratories and compared. The dyes methylene blue, 2,3,5-triphenyltetrazolium chloride, orange-O, and bromcresol purple were incorporated separately into agar used for the disc assay to determine if sharper, clearer zones could be produced. None of the dyes had an observable effect on zone size or clarity. Using the Difco disc assay, quantitation of penicillin, cephapirin, and oxytetracycline was accomplished with reliable precision by relating the size of the zone of inhibition to the log of the antibiotic concentration. The Delvotest-P and Difco disc assays were equal in sensitivity, as were spore suspensions used from the two laboratories.
采用了Delvotest - P和嗜热脂肪芽孢杆菌迪夫科纸片扩散法检测程序，并测试了检测各种抗生素的检测灵敏度。纸片扩散法中使用的嗜热脂肪芽孢杆菌孢子购自两个实验室并进行了比较。将亚甲蓝、2,3,5 - 三苯基氯化四氮唑、橙黄 - O和溴甲酚紫分别加入用于纸片扩散法的琼脂中，以确定是否能产生更清晰、更明显的抑菌圈。这些染料对抑菌圈大小或清晰度均无明显影响。使用迪夫科纸片扩散法，通过将抑菌圈大小与抗生素浓度的对数相关联，可实现对青霉素、头孢匹林和土霉素的可靠精确定量。Delvotest - P和迪夫科纸片扩散法在灵敏度上相当，两个实验室使用的孢子悬液也是如此。
