Wang Xinru, Zhang Jie, Shan Jinyu, Yang Dong, Tian Guangming, Dang Yunzhi, Ma Jianhua
School of Materials Science and Engineering, Xi'an Polytechnic University, Xi'an, Shaanxi 710048, China.
Department of Radiation Oncology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi 710086, China.
Int J Biol Macromol. 2025 Sep;322(Pt 2):146707. doi: 10.1016/j.ijbiomac.2025.146707. Epub 2025 Aug 8.
This study focuses on bacterial cellulose (BC), a material with a three-dimensional network structure, excellent biocompatibility, and superior mechanical properties, to address its inherent limitations of insufficient antibacterial activity and poor rehydration capability. Silver nanoparticles (AgNPs) were synthesized in situ on the BC surface via a hydrothermal method, followed by immersion in polyethylene glycol (PEG) and cefoperazone sodium (CEFNa) solutions to prepare BC/Ag/PEG/CEFNa composite films. The BC films and composites were comprehensively characterized using techniques such as XRD, SEM, and FTIR spectroscopy. Systematic characterization results revealed that the introduction of PEG not only increased the film's moisture absorption and water vapor permeability by 2.8 and 1.7 times, respectively, but also enhanced the CEFNa loading capacity to 1.6 times that of pure BC. Antibacterial experiments demonstrated that while pure BC films exhibited no antibacterial activity, the composite films showed inhibition zone diameters of 15.5 mm, 17.5 mm, and 12.3 mm against Escherichia coli, Staphylococcus aureus, and Pseudomonas aeruginosa, respectively. Mouse wound healing experiments indicated that the wound healing rate of the composite film treatment group was significantly improved by 21.9 % compared to the pure BC group after 15 days. These results demonstrate that the prepared BC/Ag/PEG/CEFNa composite films possess excellent physicochemical properties, significant antibacterial activity, and remarkable wound-healing effects, highlighting their great potential as advanced wound dressings for skin injury repair.
本研究聚焦于细菌纤维素（BC），这是一种具有三维网络结构、优异生物相容性和卓越机械性能的材料，旨在解决其固有的抗菌活性不足和复水能力差的局限性。通过水热法在BC表面原位合成银纳米颗粒（AgNPs），随后将其浸入聚乙二醇（PEG）和头孢哌酮钠（CEFNa）溶液中，制备BC/Ag/PEG/CEFNa复合膜。使用XRD、SEM和FTIR光谱等技术对BC膜和复合材料进行了全面表征。系统表征结果表明，PEG的引入不仅使膜的吸湿率和水蒸气透过率分别提高了2.8倍和1.7倍，还将CEFNa负载量提高到纯BC的1.6倍。抗菌实验表明，纯BC膜无抗菌活性，而复合膜对大肠杆菌、金黄色葡萄球菌和铜绿假单胞菌的抑菌圈直径分别为15.5mm、17.5mm和12.3mm。小鼠伤口愈合实验表明，15天后，复合膜治疗组的伤口愈合率比纯BC组显著提高了21.9%。这些结果表明，所制备的BC/Ag/PEG/CEFNa复合膜具有优异的理化性质、显著的抗菌活性和显著的伤口愈合效果，突出了它们作为皮肤损伤修复高级伤口敷料的巨大潜力。
