Li Shuang, Lu Chang, Zang Xiaonan, Duan Delin, Shao Zhanru
CAS and Shandong Province Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China.
Laboratory for Marine Biology and Biotechnology, Qingdao Marine Science and Technology Center, Qingdao, China.
Front Plant Sci. 2025 Jul 25;16:1640158. doi: 10.3389/fpls.2025.1640158. eCollection 2025.
Phycobiliprotein is an important co-pigment in photosynthesis, which is composed of the covalent combination of apoprotein and phycobilin. Biliverdin IXα and phycoerythrobilin are both important substances in the phycobiliprotein biosynthesis pathway. As an economic red seaweed, has a high content of phycoerythrin. Therefore, in this study, we explored new enzyme resources for the heterologous biosynthesis of biliverdin and phycoerythrobilin. Specifically, we identified and isolated the genes encoding NhHO1, NhPebA and NhPebB from , which are integral components of its phycoerythrin biosynthetic pathway. Additionally, from and from phage P-SSM2 were selected for comparative analysis. The results showed that genes from did not encode active enzymes, which might be ascribed to the absence of crucial motifs. However, the transformation of and into could lead to the synthesis of biliverdin and phycoerythrobilin. This is the first report of sequence analysis and enzyme activity verification of phycoerythrin synthesis genes from , providing a foundation for future explorations into its potential genetic resources. The successful production of biliverdin and phycoerythrobilin lay a foundation for the environmentally friendly preparation of phycobiliprotein.
藻胆蛋白是光合作用中一种重要的辅助色素，它由脱辅基蛋白和藻胆素共价结合而成。胆绿素IXα和藻红胆素都是藻胆蛋白生物合成途径中的重要物质。作为一种经济红藻，[此处原文缺失红藻名称]含有高含量的藻红蛋白。因此，在本研究中，我们探索了用于胆绿素和藻红胆素异源生物合成的新酶资源。具体而言，我们从[此处原文缺失红藻名称]中鉴定并分离了编码NhHO1、NhPebA和NhPebB的基因，它们是其藻红蛋白生物合成途径的组成部分。此外，还选择了来自[此处原文缺失来源]的[此处原文缺失物质]和来自噬菌体P - SSM2的[此处原文缺失物质]进行比较分析。结果表明，来自[此处原文缺失来源]的基因不编码活性酶，这可能归因于关键基序的缺失。然而，将[此处原文缺失物质]和[此处原文缺失物质]转化到[此处原文缺失受体]中可导致胆绿素和藻红胆素的合成。这是关于[此处原文缺失红藻名称]藻红蛋白合成基因序列分析和酶活性验证的首次报道，为未来探索其潜在遗传资源奠定了基础。胆绿素和藻红胆素的成功生产为藻胆蛋白的环境友好制备奠定了基础。
