Chen Jianmei, Zhou Nengjing, Huang Xin, Sun Zhongyu, Ding Yongqi
Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, College of Chemistry and Chemical Engineering, Hubei Normal University, Huangshi, 435002, PR China.
School of Chemistry & Materials Science, Jiangsu Normal University, 101 Shanghai Road, Xuzhou, 221116, China.
Mikrochim Acta. 2025 Aug 11;192(9):580. doi: 10.1007/s00604-025-07428-y.
Metal-organic frameworks (MOFs) are often acted as potential candidates for the construction of efficient nanozymes due to their unique metal-ligand assembly structure, which can mimic different enzyme activities by modulating the metal nodes and ligand environment. In this study, a three-in-one composite metal-organic framework nanozymes (CeCu-MOFs) with excellent intrinsic peroxidase-like, oxidase-like, and catalase-like catalytic properties have been successfully prepared in environmentally friendly manner by a one-step chemical deposition method. Meanwhile, a catalytic platform for signal amplification was designed for quantitative measurement of ascorbic acid (AA) using the oxidase-like characteristic of CeCu-MOFs. The constructed colorimetric sensor achieved selective identification and accurate measurement of AA, demonstrated good linearity in the range 0.1-50 μM with a detection limit of 0.063 μM. We, further developed a portable smartphone-based platform for visual colorimetric determination of AA in real samples in point-of-care (POC) fashion by utilizing a portable paper-based device.
金属有机框架材料（MOFs）因其独特的金属-配体组装结构，常被视为构建高效纳米酶的潜在候选材料，这种结构可以通过调节金属节点和配体环境来模拟不同的酶活性。在本研究中，通过一步化学沉积法，以环境友好的方式成功制备了具有优异的类过氧化物酶、类氧化酶和类过氧化氢酶催化性能的三合一复合金属有机框架纳米酶（CeCu-MOFs）。同时，利用CeCu-MOFs的类氧化酶特性，设计了一种用于信号放大的催化平台，用于定量测定抗坏血酸（AA）。构建的比色传感器实现了对AA的选择性识别和准确测量，在0.1-50 μM范围内表现出良好的线性，检测限为0.063 μM。我们进一步开发了一种基于便携式智能手机的平台，通过使用便携式纸质设备，以即时检测（POC）方式对实际样品中的AA进行视觉比色测定。
