利用镰叶吊灯树（Wall.ex DC.）Seem.叶提取物绿色合成氧化铜纳米颗粒及其抗氧化、抗菌、抗糖尿病和抗癌潜力评估

Green Synthesis of Copper Oxide Nanoparticles using Dolichandrone falcata (Wall.ex DC.) Seem. Leaf Extract and Assessment of Antioxidant, Antimicrobial, Anti-diabetic and Anticancer Potentials.

作者信息

Aysha MohamadYusuff, Selvam Kuppusamy, Adhavan Ramachandran, Prakash Palanisamy, Shivakumar Muthugounder Subaramanian

机构信息

Department of Botany, Periyar University, Salem, Tamil Nadu, 636 011, India.

Department of Biotechnology, Periyar University, Salem, Tamil Nadu, 636 011, India.

出版信息

Appl Biochem Biotechnol. 2025 Aug 11. doi: 10.1007/s12010-025-05356-4.

DOI:10.1007/s12010-025-05356-4

PMID:40788618

Abstract

Phyto-synthesis of Copper Oxide Nanoparticles (CuO NPs) was carried out using Dolichandrone falcata leaves. The NPs were characterised using Ultraviolet-Visible spectroscopy (UV), UV-Vis diffuse reflectance spectroscopy (UV-DRS), X-ray diffraction analysis (XRD), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and dynamic light scattering (DLS). The synthesised CuO NPs UV-Vis spectra revealed that a high absorption peak at 380 nm with optical band gap was 2.8 eV. The appearance of distinctive peaks in XRD pattern verified that CuO NPs were crystalline. Particle size distribution (DLS) revealed the average particle size 222 nm with Polydispersity Index (PDI) value is 0.348. The antibacterial ability of the synthesized CuO NPs was then investigated against major pathogens, including Gram-negative bacteria (Klebsiella pneumoniae and Pseudomonas aeruginosa) and Gram-positive bacteria (Staphylococcus aureus and Bacillus subtilis). Antioxidant activity against DPPH 61.10% ± 0.90 IC was 273.56 μg/mL, HO 64.36 ± 0.61% IC was 145.43 μg/mL and metal chelating activity 68.20% ± 0.91 with IC value 150.50 µg/mL. Anti-diabetic activity of α-amylase inhibition 75.30% ± 0.59 with IC value 725.32 µg/mL and α-glucosidase inhibition 73.21% ± 0.45 with IC value 540.40 µg/mL. Furthermore, the nanoparticle demonstrated anticancer effects with an IC of 156.26 µg/mL against the skin cancer cell line A431.

摘要

使用猫须草叶进行了氧化铜纳米颗粒（CuO NPs）的植物合成。通过紫外可见光谱（UV）、紫外可见漫反射光谱（UV-DRS）、X射线衍射分析（XRD）、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FT-IR）和动态光散射（DLS）对纳米颗粒进行了表征。合成的CuO NPs的紫外可见光谱显示，在380 nm处有一个高吸收峰，光学带隙为2.8 eV。XRD图谱中独特峰的出现证实了CuO NPs是晶体。粒度分布（DLS）显示平均粒径为222 nm，多分散指数（PDI）值为0.348。然后研究了合成的CuO NPs对主要病原体的抗菌能力，包括革兰氏阴性菌（肺炎克雷伯菌和铜绿假单胞菌）和革兰氏阳性菌（金黄色葡萄球菌和枯草芽孢杆菌）。对DPPH的抗氧化活性为61.10%±0.90，IC为273.56 μg/mL；对HO的抗氧化活性为64.36±0.61%，IC为145.43 μg/mL；金属螯合活性为68.20%±0.91，IC值为150.50 μg/mL。α-淀粉酶抑制的抗糖尿病活性为75.30%±0.59，IC值为725.32 μg/mL；α-葡萄糖苷酶抑制的抗糖尿病活性为73.21%±0.45，IC值为540.40 μg/mL。此外，该纳米颗粒对皮肤癌细胞系A431显示出抗癌作用，IC为156.26 μg/mL。