Department Biotechnology and Bioinformatics, Yogi Vemana University, Kadapa, Andhra Pradesh, India, 516005.
Department of Medical Laboratories, College of Applied Medical Sciences, Qassim University, Buraydah, Qassim, 51452, Kingdom of Saudi Arabia.
Appl Biochem Biotechnol. 2022 Oct;194(10):4424-4438. doi: 10.1007/s12010-022-03899-4. Epub 2022 Mar 31.
The emergence of new technologies has led to the discovery of the biological properties of nanoparticles through green approach. In the present investigation, we report the potential antibacterial, antioxidant, and anti-diabetic properties of copper nanoparticle (CuNPs) synthesized by reducing 3 mM copper acetate solution with aqueous leaf extract of Cocculus hirsutus. A colour change from deep brown to dark greenish brown indicated the formation of copper nanoparticles. The so-formed CuNPs were characterized by employing UV spectroscopy, FTIR, SEM, and EDX analyses which described sheet-like structure morphology having typical size of 63.46 nm. Later, the synthesized CuNPs efficiency was evaluated against bacterial pathogens, and was found highly toxic to B. subtilis and S. aureus strains. The synthesized CuNPs were examined through HO and PMA assays which demonstrated the highest free radical scavenging activity. Besides, the resulted CuNPs revealed the higher anti-diabetic efficacy in both the [Formula: see text]-amylase and [Formula: see text] -glucosidase inhibition assays (64.5% ± 0.11 and 68.5% ± 0.11, respectively). Finally, our findings report that C. hirsutus can be exploited as a source for green synthesis of CuNPs, having potent in vitro antioxidant, antibacterial, and anti-diabetic properties.
新技术的出现使得通过绿色方法发现纳米粒子的生物特性成为可能。在本研究中，我们报道了通过用 3mM 醋酸铜溶液还原 Cocculus hirsutus 的水提叶提取物合成的铜纳米粒子（CuNPs）的潜在抗菌、抗氧化和抗糖尿病特性。从深褐色到深绿褐色的颜色变化表明形成了铜纳米粒子。形成的 CuNPs 通过使用 UV 光谱、FTIR、SEM 和 EDX 分析进行了表征，这些分析描述了具有典型尺寸为 63.46nm 的片状结构形态。随后，评估了合成的 CuNPs 对细菌病原体的效率，发现其对枯草芽孢杆菌和金黄色葡萄球菌菌株具有高度毒性。通过 HO 和 PMA 测定法对合成的 CuNPs 进行了测试，结果表明其具有最高的自由基清除活性。此外，所得到的 CuNPs 在 [Formula: see text]-淀粉酶和 [Formula: see text] -葡萄糖苷酶抑制测定中表现出更高的抗糖尿病功效（分别为 64.5%±0.11 和 68.5%±0.11）。最后，我们的研究结果表明，C. hirsutus 可被用作绿色合成 CuNPs 的来源，具有潜在的体外抗氧化、抗菌和抗糖尿病特性。
