Murata Motoki, Marugame Yuki, Yamada Shuhei, Lin I-Chian, Yamashita Shuya, Kumazoe Motofumi, Fujimura Yoshinori, Tachibana Hirofumi
Advanced Research Support Center (ADRES), Ehime University, Ehime, Japan.
Division of Applied Biological Chemistry, Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka, Japan.
Sci Rep. 2025 Aug 11;15(1):29304. doi: 10.1038/s41598-025-15588-3.
Delphinidin is a major anthocyanidin belonging to the flavonoid family with pharmacological activities against cancer, inflammation, and muscle atrophy. However, the mechanism regulating its activity is yet to be completely elucidated. The present study aimed to identify the molecules responsible for the effects of delphinidin. We investigated the effects of delphinidin on melanoma cells and observed that delphinidin upregulates the expression of let-7b, a microRNA (miRNA) that inhibits the proliferation of melanoma cells. Delphinidin-induced upregulation of let-7b led to the suppression of cyclin D1, a target gene associated with cell cycle progression. Using genetic functional screening, we identified that family with sequence similarity 222 member B (Fam222B) is an important factor behind the inhibitory effects of delphinidin against melanoma cell proliferation and that the transfection with small interfering RNA specifically targeting Fam222B altered miRNAs expression in B16 cells. In summary, delphinidin exerts antimelanoma effects by increasing let-7b expression through Fam222B, and Fam222B is involved in regulating miRNA expression.
飞燕草素是一种主要的花青素，属于黄酮类化合物，具有抗癌、抗炎和抗肌肉萎缩的药理活性。然而，调节其活性的机制尚未完全阐明。本研究旨在确定负责飞燕草素作用的分子。我们研究了飞燕草素对黑色素瘤细胞的影响，观察到飞燕草素上调了let-7b的表达，let-7b是一种抑制黑色素瘤细胞增殖的微小RNA（miRNA）。飞燕草素诱导的let-7b上调导致细胞周期蛋白D1的抑制，细胞周期蛋白D1是与细胞周期进程相关的靶基因。通过基因功能筛选，我们确定序列相似性家族222成员B（Fam222B）是飞燕草素抑制黑色素瘤细胞增殖作用背后的一个重要因素，并且用特异性靶向Fam222B的小干扰RNA转染会改变B16细胞中的miRNA表达。总之，飞燕草素通过Fam222B增加let-7b表达发挥抗黑色素瘤作用，并且Fam222B参与调节miRNA表达。
