Shabaka Fatma H, Rashed Laila A, Ali Mona S, Salama Aya A
Department of Dermatology and Venereology, Faculty of Medicine for Girls, Al-Azhar University, Cairo, Egypt.
Department of Biochemistry, Faculty of Medicine-Cairo University, Cairo, Egypt.
Dermatol Pract Concept. 2025 Jul 31;15(3):5027. doi: 10.5826/dpc.1503a5027.
Rosacea is a chronic inflammatory skin disorder distinguished by recurrent episodes of inflammatory papules, persistent erythema, facial flushing, pustules, and telangiectasia. Any disturbance in the gut microbiome could influence the immune system equilibrium in rosacea by releasing zonulin, leading to increased intestinal permeability and the passage of many microbes into the circulation, causing inflammation.
We aimed to estimate the serum zonulin levels in cases with rosacea compared with healthy controls. Some fecal bacteria were investigated in an attempt to find a relationship between gut microbiome and rosacea.
This case-control study was performed on 42 participants aged above 18 years: 21 patients with a clinical diagnosis of rosacea approved by dermoscopy and 21 healthy individuals as controls. The serum zonulin level was estimated by the enzyme-linked immunosorbent assay technique, and some gut microbiomes were investigated using real-time quantitative PCR.
There was a statistically significantly higher serum zonulin level in rosacea cases than in controls. There was a statistically significant elevation in Bacteroides and Lactobacillus gut microbiomes in rosacea patients compared to controls, while there was no statistically significant increase in Fusobacterium microbiome in patients. Zonulin levels did not show a significant correlation with gut microbiome.
Serum zonulin measurement can be used as a discriminating marker between rosacea and healthy controls, due to getting a specific cut-off point in ROC analysis with the highest specificity and sensitivity (100% and 100%, respectively). Gut microbial dysbiosis could play a valuable role in the disease pathogenesis.
酒渣鼻是一种慢性炎症性皮肤病，其特征为炎症性丘疹反复发作、持续性红斑、面部潮红、脓疱和毛细血管扩张。肠道微生物群的任何紊乱都可能通过释放闭合蛋白影响酒渣鼻的免疫系统平衡，导致肠道通透性增加，许多微生物进入循环系统，引发炎症。
我们旨在评估酒渣鼻患者与健康对照者的血清闭合蛋白水平。对一些粪便细菌进行研究，试图找出肠道微生物群与酒渣鼻之间的关系。
本病例对照研究对42名18岁以上的参与者进行：21例经皮肤镜检查临床诊断为酒渣鼻的患者和21名健康个体作为对照。采用酶联免疫吸附测定技术评估血清闭合蛋白水平，使用实时定量PCR研究一些肠道微生物群。
酒渣鼻患者的血清闭合蛋白水平在统计学上显著高于对照组。与对照组相比，酒渣鼻患者的拟杆菌属和乳杆菌属肠道微生物群有统计学显著升高，而患者的梭杆菌属微生物群没有统计学显著增加。闭合蛋白水平与肠道微生物群无显著相关性。
血清闭合蛋白测量可作为酒渣鼻与健康对照之间的鉴别标志物，因为在ROC分析中获得了具有最高特异性和敏感性（分别为100%和100%）的特定截断点。肠道微生物失调可能在疾病发病机制中发挥重要作用。
