Kılıç Yakup, Dalkılıç Semih, Kadıoğlu Dalkılıç Lütfiye, Özdemir Emrah, Uğurlu Fatih Mehmet, Pala Ragıp, Ayyıldız Yeliz, Hepsert Sezgin, Ayılgan Ercan
Faculty of Sport Sciences, Firat University, Elazig, Turkey.
Faculty Of Science / Molecular Biology, Firat University, Elazig, Turkey.
Diabetol Metab Syndr. 2025 Aug 11;17(1):324. doi: 10.1186/s13098-025-01893-4.
Type 2 diabetes mellitus (T2DM) is a common metabolic disease characterized by elevated blood glucose levels accompanied by inadequate insulin secretion. In this study, we aimed to determine the expression levels of PEPCK, FAS, INSIG-1 and TNF-α genes, which play a key role in metabolism and inflammation processes, in brain tissue of rats with streptozotocin (STZ)-induced diabetes and to investigate how these gene expressions are affected by different exercise protocols.
In this study, 40 male Wistar albino rats were randomly divided into four groups: Control group (C), Diabetes Sedentary group (DS), Diabetes Heavy Exercise group (DHE) and Diabetes Light Exercise group (DLE). Diabetes was induced by a single dose of intraperitoneally administered streptozotocin (STZ, 60 mg/kg). After diabetes induction, rats were subjected to treadmill exercise 5 days a week for 6 weeks. 24 h after the last exercise session, rats were sacrificed by decapitation and brain tissues were sent to the laboratory for molecular analysis.
A significant decrease in FAS (p = 0.002) and TNF-α (p = 0.044) levels was observed in the DHE group, while a significant increase in PEPCK (p = 0.009) and INSIG1 (p = 0.019) levels and a significant decrease in FAS (p = 0.037) and TNF-α (p = 0.014) levels were found in the DLE group. In addition, blood glucose levels were significantly decreased in the exercise groups compared to the diabetic sedentary group (p = 0.001).
This study revealed that exercise protocols of different intensities induce significant changes in the expression levels of some metabolic and inflammatory genes in brain tissue and decrease blood glucose levels in rats with streptozotocin-induced diabetes model. The findings suggest that exercise may partially modulate the molecular processes in the central nervous system of diabetes and may offer potential therapeutic contributions.
2型糖尿病（T2DM）是一种常见的代谢性疾病，其特征为血糖水平升高并伴有胰岛素分泌不足。在本研究中，我们旨在测定在链脲佐菌素（STZ）诱导的糖尿病大鼠脑组织中，对代谢和炎症过程起关键作用的磷酸烯醇式丙酮酸羧激酶（PEPCK）、脂肪酸合酶（FAS）、胰岛素诱导基因1（INSIG-1）和肿瘤坏死因子-α（TNF-α）基因的表达水平，并研究不同运动方案如何影响这些基因的表达。
在本研究中，40只雄性Wistar白化大鼠被随机分为四组：对照组（C）、糖尿病久坐组（DS）、糖尿病高强度运动组（DHE）和糖尿病低强度运动组（DLE）。通过单次腹腔注射链脲佐菌素（STZ，60mg/kg）诱导糖尿病。糖尿病诱导后，大鼠每周进行5天跑步机运动，持续6周。最后一次运动后24小时，大鼠断头处死，脑组织送至实验室进行分子分析。
在DHE组中观察到FAS（p = 0.002）和TNF-α（p = 0.044）水平显著降低，而在DLE组中发现PEPCK（p = 0.009）和INSIG1（p = 0.019）水平显著升高，FAS（p = 0.037）和TNF-α（p = 0.014）水平显著降低。此外，与糖尿病久坐组相比，运动组的血糖水平显著降低（p = 0.001）。
本研究表明，不同强度的运动方案可诱导链脲佐菌素诱导的糖尿病大鼠脑组织中某些代谢和炎症基因的表达水平发生显著变化，并降低血糖水平。研究结果表明，运动可能部分调节糖尿病中枢神经系统中的分子过程，并可能提供潜在的治疗作用。
