仓鼠细胞，未处理的以及用化学致癌物处理过的，在体外培养了2年半。
Hamster cells, untreated and treated with chemical carcinogens, maintained in vitro for 2 1/2 years.
作者信息
Papadopoulo D, Levy S, Chamaillard L, Beesau O, Hubert-Harbart M, Markovits P
出版信息
Br J Cancer. 1977 Jul;36(1):65-71. doi: 10.1038/bjc.1977.155.
We have maintained in culture, for a prolonged period, untreated hamster cells from whole embryo, foetal brain and lung from newborn animals. Among the 7 lines studied we observed only one spontaneous transformation during the first year of culture. The cells of the 6 other control lines remained normal and diploid, and were not transplantable during the first 9 to 12 months of culture. After the 12th month, changes appeared in their in vitro behaviour and their transplantability: grafts of 0-5-2 X 10(6) cells induced tumours in the hamster; fewer cells did not. In vitro chemically transformed hamster cells were fundamentally different from untreated cells of the same origin, not only in morphological and growth characteristics but also in transplantability; of the 9 lines obtained, 7 induced tumours after injection of 10(1)-10(4) cells, and 2 after injection of 10(5) cells per animal.
我们长期培养新生动物全胚胎、胎儿脑和肺的未经处理的仓鼠细胞。在所研究的7个细胞系中，我们在培养的第一年仅观察到1次自发转化。其他6个对照细胞系的细胞在培养的前9至12个月内保持正常和二倍体状态，并且不可移植。12个月后，它们的体外行为和移植能力出现了变化：移植0 - 5 - 2×10⁶个细胞可在仓鼠体内诱发肿瘤；细胞数量较少则不会。体外化学转化的仓鼠细胞与相同来源的未经处理的细胞在根本上不同，不仅在形态和生长特征方面，而且在移植能力上也不同；在所获得的9个细胞系中，7个在每只动物注射10¹ - 10⁴个细胞后诱发肿瘤，2个在每只动物注射10⁵个细胞后诱发肿瘤。
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