Progestogens, androgens and their glucuronides in the terminal stages of oocyte maturation in landlocked Atlantic salmon.

Peripheral serum levels of free and conjugated steroids were correlated with seven terminal stages of oocyte maturation in female landlocked Atlantic salmon. Pregnenolone levels were maximal at stage 1, and there was a surge in testosterone after initiation of germinal vesicle migration. Testosterone remained high, above 25 ng/ml until ovulation, and its glucuronide was always lower than the corresponding free form. 17 alpha,20 beta-Dihydroxy-4-pregnen-3-one increased progressively from 0.71 to 68 ng/ml from oocyte stage 1 to 6, with the glucuronide predominating in stages 1 to 4 and the free steroid in stages 5 to 7. Thus, very low levels of free 17 alpha,20 beta-dihydroxy-4-pregnen-3-one were present when migration of the germinal vesicle was initiated but considerable free steroid was present at and after germinal vesicle breakdown. Negligible amounts of progesterone, 17 alpha-hydroxypregnenolone, 5-pregnene-3 beta,17 alpha,20 beta-triol were found in the serum during final maturation. In male salmon with expressible milt, 17 alpha,20 beta-dihydroxy-4-pregnen-3-one was present with a gradual increase of both free and conjugated form at the advent of spawning, suggesting that this steroid may also play a role in reproduction in male fish. The action of steroids in vitro on oocytes at various stages was consistent with conclusions based on blood levels.