Pine J
J Neurosci Methods. 1980 Feb;2(1):19-31. doi: 10.1016/0165-0270(80)90042-4.
Dissociated cell cultures of neurons from neonatal rat superior cervical ganglia have been grown in specially prepared dishes, the bottoms of which consist of glass coverslips on which thin-film microcircuits have been deposited. The microcircuit provides 32 microelectrodes per dish, each approximately 8 X 10 micrometer in area. Extracellular recordings of action potentials from individual neurons have been made, with good signal-to-noise ratios, for cells within 40 micrometer of the electrode centers. The microelectrodes are also suitable for passing the current required for extracellular stimulation and action potentials have been evoked by stimulating cell bodies and processes through the electrodes.
新生大鼠颈上神经节神经元的解离细胞培养物已在特制培养皿中生长，培养皿底部由已沉积薄膜微电路的玻璃盖玻片组成。每个培养皿中的微电路提供32个微电极，每个微电极的面积约为8×10微米。已对电极中心40微米范围内的单个神经元的动作电位进行了细胞外记录，信噪比良好。这些微电极也适用于通过细胞外刺激所需的电流，并且通过电极刺激细胞体和神经突已诱发了动作电位。
