Barritault D, Hayes D H
Biochimie. 1977;59(5-6):463-72. doi: 10.1016/s0300-9084(77)80051-5.
Analyses of the T1 ribonuclease-alkaline phosphatase fingerprint of a continuous fragment of the 16S rRNA, 170-230 nucleotides long, isolated from the products of autodigestion of 30S ribosome subunits show that it contains a sequence near the 5'-phosphate terminus of intact 16S rRNA and corresponds to segment H'-M of this molecule as defined by Ehresmann et al [29]. Incubation of this fragment with total 30S ribosomal proteins under reconstitution conditions leads to the formation of a complex containing proteins S4, S20, and one or both of proteins S16 and S17. The stoichiometry of these proteins in the complex is discussed.
对从30S核糖体亚基自消化产物中分离出的一段长度为170 - 230个核苷酸的16S rRNA连续片段的T1核糖核酸酶 - 碱性磷酸酶指纹图谱分析表明，它在完整16S rRNA的5'-磷酸末端附近包含一个序列，并且对应于Ehresmann等人[29]所定义的该分子的H'-M片段。在重组条件下将该片段与总的30S核糖体蛋白一起温育会导致形成一种复合物，该复合物包含蛋白S4、S20以及蛋白S16和S17中的一种或两种。讨论了这些蛋白在复合物中的化学计量。
