Clinopodium barosmum (C. barosmum), a rare species within the genus Clinopodium of the Lamiaceae family in northwestern Yunnan, is highly valued for its exceptional ecological and medicinal properties. This study presents the successful assembly of the chromosome-level genome of C. barosmum utilizing Nanopore sequencing technology and chromosome conformation capture techniques. The assembled genome spans 518.59 Mb, with a scaffold N50 length of 21.12 Mb. Notably, 99.97% of the genomic data (518.42 Mb) was accurately anchored and assigned to 24 chromosomes. Analysis revealed a high proportion of repetitive sequences, comprising approximately 51.81% of the genome, and identified 41,864 protein-coding genes. Furthermore, a comparative genomic analysis was conducted, establishing a robust foundation and providing valuable reference data for future investigations of C. barosmum.